PDGFR alpha Antibody (JF104-6)

Novus Biologicals | Catalog # NBP2-67025

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF104-6 expressed in HEK293
View all PDGF R alpha Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within C-terminal human PDGFR alpha. (SwissProt: P16234 Human; SwissProt: P26618 Mouse; SwissProt: P20786 Rat)

Localization

Golgi apparatus, Cell membrane, cilium.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGFR alpha Antibody (JF104-6)

Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Western blot analysis of PDGFR alpha on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.
Flow Cytometry: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Flow Cytometry: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Flow Cytometry: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of NIH/3T3 cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Western Blot: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025]

Immunohistochemistry-Paraffin: PDGFR alpha Antibody (JF104-6) [NBP2-67025] - Analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.

Applications for PDGFR alpha Antibody (JF104-6)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDGF R alpha

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (JF104-6)

Certificate of Analysis

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Product Specific Notices for PDGFR alpha Antibody (JF104-6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDGFR alpha Antibody (JF104-6)

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Protocols

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