Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

Novus Biologicals | Catalog # NBP2-37300

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C5H8

Format

BSA Free
View all Perilipin-2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Perilipin-2/ADFP (AA: 286-437) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mouse mAb against HepG2 cell lysate.
Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunocytochemistry/ Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunocytochemistry/Immunofluorescence: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Immunofluorescence analysis of HepG2 cells using PLIN2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of bladder cancer tissues using PLIN2 mouse mAb with DAB staining.
Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Flow Cytometry: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of HepG2 cells using PLIN2 mouse mAb (green) and negative control (red).
Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against human PLIN2 (AA: 286-437) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8)BSA Free [NBP2-37300]

Western Blot: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis using PLIN2 mAb against HEK293 (1) and PLIN2 (AA: 286-437)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

Immunohistochemistry-Paraffin: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Analysis of rectum cancer tissues using PLIN2 mouse mAb with DAB staining.
ELISA: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

ELISA: Perilipin-2/ADFP Antibody (2C5H8) - BSA Free [NBP2-37300]

ELISA: Perilipin-2/ADFP Antibody (2C5H8) [NBP2-37300] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin-2

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

Alternate Names

ADFP, Adipophilin, Perilipin2, PLIN2

Gene Symbol

PLIN2

Additional Perilipin-2 Products

Product Documents for Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Perilipin-2/ADFP Antibody (2C5H8) - BSA Free

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Protocols

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