Perilipin-3/TIP47 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87871

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLGKLRATKQRAQEALLQLSQALSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Perilipin-3/TIP47 Antibody - BSA Free

Perilipin-3/TIP47 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody - BSA Free [NBP1-87871]

Immunocytochemistry/ Immunofluorescence: Perilipin-3/TIP47 Antibody - BSA Free [NBP1-87871]

Staining of human cell line A-431 shows localization to lipid droplets.
Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871]

Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Perilipin-3/TIP47 Antibody - BSA Free Western Blot: Perilipin-3/TIP47 Antibody - BSA Free [NBP1-87871]

Western Blot: Perilipin-3/TIP47 Antibody - BSA Free [NBP1-87871]

Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PLIN3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for Perilipin-3/TIP47 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87871 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin-3

TIP47 has been described as cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. PLIN3 has been localized in milk fat globule membranes of human and bovine origin. It has also been described as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients. Therefore, TIP47 is probably significant as an oncodevelopmental marker.

Alternate Names

M6PRBP1, Perilipin3, PLIN3, PP17, TIP47

Gene Symbol

PLIN3

Additional Perilipin-3 Products

Product Documents for Perilipin-3/TIP47 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Perilipin-3/TIP47 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Perilipin-3/TIP47 Antibody - BSA Free

Customer Reviews for Perilipin-3/TIP47 Antibody - BSA Free (1)

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  • Perilipin-3/TIP47 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: hela cell
    Species: Human
    Verified Customer | Posted 02/19/2019
    See other experiment details for caption
    Western blot analysis of cell lysates from HeLa cells transfected with pCi-3xFlag plasmids expressing human Perilipin 3 (PLIN3), human Perilipin 2 (PLIN2), empty vector (mock), and human Vastus lateralis muscle samples (muscle). Proteins were revealed using ABIN4344877 (top) and an anti-Flag-tag antibody (middle) for endogenous and/or recombinant, Flag-tagged PLIN3 and PLIN2. GAPDH (bottom) revealed using a GAPDH antibody served as loading control. The PLIN3 antibody ABIN4344877 reveals a protein of the expected molecular weight of the recombinant PLIN3 in lysates of transfected HeLa cells. It does also detect endogenous PLIN3 in extracts of mock-transfected Hela cells and human muscle tissue. *https://www.antibodies-online.com/antibody/4344877/anti-Perilipin+3+PLIN3+antibody/#tab-validation-566
    Perilipin-3/TIP47 Antibody - BSA Free NBP1-87871

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