Perilipin-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13776

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Perilipin-4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: GVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Perilipin-4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue, breast, colon and liver using Anti-Perilipin-4 antibody NBP2-13776 (A) shows similar protein distribution across tissues to independent antibody NBP2-38731 (B).
Western Blot: Perilipin-4 Antibody [NBP2-13776]

Western Blot: Perilipin-4 Antibody [NBP2-13776]

Western Blot: Perilipin-4 Antibody [NBP2-13776] - Analysis in human adipose tissue.
Immunocytochemistry/ Immunofluorescence: Perilipin-4 Antibody [NBP2-13776]

Immunocytochemistry/ Immunofluorescence: Perilipin-4 Antibody [NBP2-13776]

Immunocytochemistry/Immunofluorescence: Perilipin-4 Antibody [NBP2-13776] - Staining of human cell line RT4 shows localization to plasma membrane, cytosol & lipid droplets. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human breast shows moderate membranous positivity in adipocytes.
Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue shows moderate membranous positivity in adipocytes.
Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776]

Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human colon shows no positivity in glandular cells as expected.
Perilipin-4 Antibody - BSA Free

Western Blot: Perilipin-4 Antibody - BSA Free [NBP2-13776] -

Relative levels of hepatic proteins and mRNAs of PAT family genes in the male mice fed the Western diet for 3 weeks starting at 6 weeks of age. (A) Immunoblots of perilipins and control proteins. (B) Densitometry of the immunoblots in A (n = 5–6). (C) Relative levels of hepatic mRNAs for perilipins in the mice at necropsy (n = 5–6). *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32170127), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Perilipin-4 Antibody - BSA Free

Western Blot: Perilipin-4 Antibody - BSA Free [NBP2-13776] -

Deletion of CGI-58 in Huh7 human hepatoma cell line increases cellular lipids and perilipin expression. (A) The sgRNA sequence targeting Exon 3 of human CGI-58 gene and the sequencing result of the DNA from CGI-58 knockout (KO) Huh7 cells. (B) Immunoblots of the KO and control Huh7 cell lysates. (C) Fluorescence microscopic images of LDs stained with Bodipy (green) and nuclei stained with DAPI (blue) in the KO and control cells. (D) Triglyceride (TG) content in the KO and control cells (n = 5). (E) Western blots of perilipins in the KO and control cell lysates. The blots were quantified by densitometry. (F) Relative levels of mRNAs for perilipins in the KO and control cells (n = 4). GAPDH was used as an internal invariant control. *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32170127), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Perilipin-4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Perilipin-4 Antibody - BSA Free [NBP2-13776] -

Perilipins (PLIN)4 is mostly expressed in slow muscle fibers. (A) Representative images (400X) from confocal fluorescence microscopy of sections of paraffin-embedded biopsies from m. vastus lateralis (n = 10), stained with antibodies against myosin heavy chain fast (red, mouse anti-myosin heavy chain; skeletal, fast) and PLIN4 (green, guinea pig anti-PLIN4). Fast muscle fibers are stained in bright red color, whereas slow fibers appear as dark. (B) M. soleus and m. gastrocnemius were dissected from male C57BL/6N mice (n = 5) and Plin4 mRNA expression was determined by quantitative RT-PCR. Plin4 mRNA was normalized to Tbp mRNA. Results are presented as means +/- SEM, *P < 0.05 (two-tailed Student’s t-test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26265748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Perilipin-4 Antibody - BSA Free

Immunohistochemistry-Paraffin: Perilipin-4 Antibody - BSA Free [NBP2-13776] -

Intramuscular adipocytes. (A) Sections of paraffin-embedded biopsies from m. vastus lateralis (n = 8) were stained with PLIN1 antibody (red, mouse anti-PLIN1) to detect adipocytes (A). Representative images (200X) were obtained by wide-field fluorescence microscopy. High magnification (1000X) of adipocytes is shown in the insert. (B) The sections were stained for PLIN4 (green, rabbit anti-PLIN4) and PLIN1 (red, mouse anti-PLIN1), showing partly co-localization in intramuscular adipocytes (n = 4). The merged image (200X) shows co-localization of the proteins (white arrow). The image was obtained by confocal fluorescence microscopy. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26265748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Perilipin-4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP2-13776 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin-4

Perilipin-4 may play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in thebiogenesis of lipid droplets.

Alternate Names

KIAA1881, MDRV, MRUPAV, NIPA2, S3-12

Gene Symbol

PLIN4

Additional Perilipin-4 Products

Product Documents for Perilipin-4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Perilipin-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Perilipin-4 Antibody - BSA Free

Customer Reviews for Perilipin-4 Antibody - BSA Free (1)

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Customer Images

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  • Perilipin-4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Skeletal muscle
    Species: Pig
    Verified Customer | Posted 09/15/2020
    Detection of PLIN4 in pig muscle cross section dilution 1:200 in PBS with 2% NGS secondary antibody 1:1000 Alexa Fluor 488 goat anti-rabbit IgG
    no fluorescence was detected when blocking the antibody binding in advance with the blocking peptide
    Perilipin-4 Antibody - BSA Free NBP2-13776

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Protocols

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FAQs for Perilipin-4 Antibody - BSA Free

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  • Q: Can you please provide me the concentration of the lot A96877?

    A: I can confirm that lot number A96877 is supplied at 0.2mg/ml.

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