Perilipin Antibody (1K4W3)

Novus Biologicals | Catalog # NBP3-16480

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1K4W3 expressed in HEK293
View all Perilipin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin (O60240). HYVPLPRLSLMEPESEFRDIDNPPAEVERREAERRASGAPSAGPEPAPRLAQPRRSLRSAQSPGAPPGPGLEDEVATPAAPRPGFPAVPREKPKRRVSDSF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Perilipin Antibody (1K4W3)

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480]

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480]

Western Blot: Perilipin Antibody (1K4W3) [NBP3-16480] - Western blot analysis of extracts of various cell lines, using Perilipin Rabbit mAb (NBP3-16480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded rat brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480]

Immunohistochemistry-Paraffin: Perilipin Antibody (1K4W3) [NBP3-16480] - Immunohistochemistry of paraffin-embedded mouse brown adipose using Perilipin Rabbit mAb (NBP3-16480) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Perilipin Antibody (1K4W3)

Immunocytochemistry/ Immunofluorescence: Perilipin Antibody (1K4W3) [Perilipin] -

Immunocytochemistry/ Immunofluorescence: Perilipin Antibody (1K4W3) [Perilipin] - Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for Perilipin Antibody (1K4W3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Perilipin

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody (1K4W3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Perilipin Antibody (1K4W3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

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