Perilipin Antibody [FITC]

Novus Biologicals | Catalog # NB110-40760F

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Fish

Predicted:

Bovine (92%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Imaging Mass Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Perilipin Primary Antibodies »

Product Specifications

Immunogen

This Perilipin Antibody was developed against a synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 27012897).

Localization

Endoplasmic reticulum, Lipid droplet (surface-associated).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Perilipin Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Imaging Mass Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Perilipin

Perilipin is a member of the perilipin or PAT family which includes perilipin or perilipin 1 (PLIN1), ADRP/perilipin 2, TIP47/perilipin 3, S3-12/perilipin 4, and LSDP5/perilipin 5 (also called OXPAT). PLIN1 is localized on lipid droplet surfaces in adipocytes and steroidogenic cells wherein it plays a key role in basal/stimulated lipid storage as well as breakdown. Under basal conditions, perilipin protects the lipid core from lipases (e.g. hormone-sensitive lipase and adipocyte TAG lipase) and co-activator CGI-58 by sequestering the enzymes from the lipid droplet surfaces. However, upon beta-adrenergic stimulation, PKA phosphorylates PLIN1 and causes the release of CGI-58 so it can bind and stimulate ATGL and also allows/recruits HSL to translocate to the lipid droplet surface. PLIN1 knockout increases basal lipolysis and decreases LD size in adipocytes and causes resistance to diet induced obesity in mice. In humans, lower PLIN1 expression is associated with higher rates of lipolysis and mutation in PLIN1 or its expression in obese human adipose tissue, correlates positively with insulin sensitivity, and have been established as the cause of familial partial lipodystrophy type 4 (FPLD4).

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Perilipin Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Perilipin Antibody [FITC]

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    • A: The FITC conjugate of this perilipin antibody, NB110-40760F, has not itself been tested in ICC/IF yet, which is why this application is not stated on its datasheet. Nonetheless, the unconjugated format of the antibody, NB110-40760, has, and so is guaranteed for ICC/IF. Our product notes for this antibody say that use in ICC/IF was reported in the literature, in this paper: http://www.ncbi.nlm.nih.gov/pubmed/18201980) Therefore, we cannot guarantee NB110-40760F for IF, but the evidence we do have does indicate that it should work for you. If you did go ahead and test NB110-40760F in IF, you would be eligible for our Innovator's Reward, where in return for sharing your findings with us (whether the antibody proved suitable or not) by completing a review, we would give you a voucher code for the price of the product (please see here for further details: Innovator's Reward program).
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