Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Fish

Cited:

Human, Mouse, Rat

Predicted:

Bovine (92%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Imaging Mass Cytometry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Perilipin Primary Antibodies »

Product Specifications

Immunogen

This Perilipin Antibody was developed against a synthetic peptide made to a region between residues 450-522 (C-terminus) of the human Perilipin protein. [Swiss-Prot# O60240]

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 27012897).

Localization

Endoplasmic reticulum, Lipid droplet (surface-associated).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Perilipin Antibody - BSA Free (NB110-40760) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Perilipin Antibody: Cited in 16 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Perilipin Antibody - BSA Free

Western Blot: Perilipin Antibody [NB110-40760]

Western Blot: Perilipin Antibody [NB110-40760]

Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/mL anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.
Flow Cytometry: Perilipin Antibody [NB110-40760]

Flow Cytometry: Perilipin Antibody [NB110-40760]

Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.
Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760]

Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.
Flow Cytometry: Perilipin Antibody [NB110-40760]

Flow Cytometry: Perilipin Antibody [NB110-40760]

Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Imaging Mass Cytometry: Perilipin Antibody [NB110-40760]

Imaging Mass Cytometry: Perilipin Antibody [NB110-40760]

Imaging Mass Cytometry: Perilipin Antibody [NB110-40760] - Human bone marrow analyzed by IMC. Perilipin antibody conjugated with 151Eu and used 1:50 on human bone marrow FFPE. Perilipin - red. DNA- white. IMC image submitted by a verified customer review.

Applications for Perilipin Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

5 - 10 ug/mL

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/mL
Application Notes
This Perilipin antibody is useful for Western blot anlaysis where a band at ~60 kDa is seen. This Perilipin Antibody is validated for Imaging Mass Cytometry from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB110-40760 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Perilipin

Perilipin is a member of the perilipin or PAT family which includes perilipin or perilipin 1 (PLIN1), ADRP/perilipin 2, TIP47/perilipin 3, S3-12/perilipin 4, and LSDP5/perilipin 5 (also called OXPAT). PLIN1 is localized on lipid droplet surfaces in adipocytes and steroidogenic cells wherein it plays a key role in basal/stimulated lipid storage as well as breakdown. Under basal conditions, perilipin protects the lipid core from lipases (e.g. hormone-sensitive lipase and adipocyte TAG lipase) and co-activator CGI-58 by sequestering the enzymes from the lipid droplet surfaces. However, upon beta-adrenergic stimulation, PKA phosphorylates PLIN1 and causes the release of CGI-58 so it can bind and stimulate ATGL and also allows/recruits HSL to translocate to the lipid droplet surface. PLIN1 knockout increases basal lipolysis and decreases LD size in adipocytes and causes resistance to diet induced obesity in mice. In humans, lower PLIN1 expression is associated with higher rates of lipolysis and mutation in PLIN1 or its expression in obese human adipose tissue, correlates positively with insulin sensitivity, and have been established as the cause of familial partial lipodystrophy type 4 (FPLD4).

Alternate Names

PERI, PLIN, PLIN1

Gene Symbol

PLIN1

Additional Perilipin Products

Product Documents for Perilipin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Perilipin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

Citations for Perilipin Antibody - BSA Free

Customer Reviews for Perilipin Antibody - BSA Free (1)

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Customer Images

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  • Perilipin Antibody
    Name: Anonymous
    Application: Imaging Mass Cytometry
    Sample Tested: bone marrow
    Species: Human
    Verified Customer | Posted 02/22/2021
    Human bone marrow analyzed by IMC. Perilipin - red DNA- white
    Conjugated with 151Eu and used 1:50 on human bone marrow FFPE. Perilipin - red DNA- white
    Perilipin Antibody - BSA Free NB110-40760
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

View specific protocols for Perilipin Antibody - BSA Free (NB110-40760):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 4% paraformaldehyde to the dish and fix at room temperature for 10 minutes.
2. Remove the paraformaldehyde and wash the cells in PBS.
3. Permeabilize the cells with 0.1% Triton X100 or other suitable detergent for 2 min.
4. Remove the permeabilization buffer and wash three times for 5 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 5 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 5 minutes each.
10. Counter stain DNA with DAPI if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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