Peroxiredoxin 3 Antibody (14E2) - BSA Free

Novus Biologicals | Catalog # NBP3-26371

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 14E2 expressed in HEK293

Format

BSA Free
View all Peroxiredoxin 3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Peroxiredoxin 3 [UniProt P30048]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Peroxiredoxin 3 Antibody (14E2) - BSA Free

Peroxiredoxin 3 Antibody (14E2)

Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] -

Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Peroxiredoxin 3 Antibody (14E2)

Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] -

Immunohistochemistry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Image of Peroxiredoxin 3 Antibody (14E2) diluted at 1:100 and staining in paraffin-embedded human lung tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Peroxiredoxin 3 Antibody (14E2)

Western Blot: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] -

Western Blot: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Positive Western Blot detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.
All lanes: Peroxiredoxin 3 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 28, 26 kDa
Observed band size: 20-28 kDa
Peroxiredoxin 3 Antibody (14E2)

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] -

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Staining of PC-3 cell with Peroxiredoxin 3 Antibody (14E2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 503-conjugated Goat Anti-Rabbit IgG (H+L).
Peroxiredoxin 3 Antibody (14E2)

Flow Cytometry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] -

Flow Cytometry: Peroxiredoxin 3 Antibody (14E2) [NBP3-26371] - Overlay Peak curve showing HepG2 cells stained with Peroxiredoxin 3 Antibody (14E2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Peroxiredoxin 3 Antibody (14E2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 3

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.

Alternate Names

AOP-1, MER5, PRDX3, SP-22

Gene Symbol

PRDX3

Additional Peroxiredoxin 3 Products

Product Documents for Peroxiredoxin 3 Antibody (14E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Peroxiredoxin 3 Antibody (14E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

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Protocols

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