PEX5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87185

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PEX5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQHTASDFVAKVDDPKLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEX5 Antibody - BSA Free

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-PEX5 antibody NBP1-87185 (A) shows similar protein distribution across tissues to independent antibody NBP2-38443 (B).
Western Blot: PEX5 Antibody [NBP1-87185]

Western Blot: PEX5 Antibody [NBP1-87185]

Western Blot: PEX5 Antibody [NBP1-87185] - Analysis using Anti-PEX5 antibody NBP1-87185 (A) shows similar pattern to independent antibody NBP2-38443 (B).
Western Blot: PEX5 Antibody [NBP1-87185]

Western Blot: PEX5 Antibody [NBP1-87185]

Western Blot: PEX5 Antibody [NBP1-87185] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PEX5 Antibody [NBP1-87185]

Immunocytochemistry/ Immunofluorescence: PEX5 Antibody [NBP1-87185]

Immunocytochemistry/Immunofluorescence: PEX5 Antibody [NBP1-87185] - Staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human lymph node using Anti-PEX5 antibody.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human cerebral cortex using Anti-PEX5 antibody.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human colon using Anti-PEX5 antibody NBP1-87185.
Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185]

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP1-87185] - Staining of human testis using Anti-PEX5 antibody NBP1-87185.

Applications for PEX5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PEX5

The product of the PEX5 gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Alternate Names

FLJ50634, FLJ50721, Peroxin-5, peroxisomal biogenesis factor 5, Peroxisomal C-terminal targeting signal import receptor, peroxisomal targeting signal 1 receptor, peroxisomal targeting signal import receptor, peroxisomal targeting signal receptor 1, Peroxisome receptor 1peroxin-5, PTS1 receptor, PTS1-BP, PTS1RFLJ51948, PXR1peroxisomal targeting signal 1 (SKL type) receptor

Entrez Gene IDs

5830 (Human)

Gene Symbol

PEX5

Additional PEX5 Products

Product Documents for PEX5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PEX5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PEX5 Antibody - BSA Free

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Protocols

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FAQs

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