PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14153

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-PHF10-2A10

Format

Azide and BSA Free
View all PHF10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length humanPHF10protein (Uniprot: Q8WUB8)

Reactivity Notes

Predicted to react in Mouse and Chick.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.05 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13750).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153]

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153]

Protein Array: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PHF10 Antibody (PCRP-PHF10-2A10).
PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Immunocytochemistry/ Immunofluorescence: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free
PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free Flow Cytometry: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Flow Cytometry: PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free [NBP3-14153] -

Flow cytometric analysis of PFA-fixed HeLa cells. PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PHF10

PHF10 contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Alternate Names

BAF45a, BAF45APHD zinc finger protein XAP135, BRG1-associated factor 45a, FLJ10975, MGC111009, PHD finger protein 10, XAP135BRG1-associated factor, 45-KD, A

Gene Symbol

PHF10

Additional PHF10 Products

Product Documents for PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PHF10 Antibody (PCRP-PHF10-2A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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