PHF23 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13756

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHF23 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: MATVVGGEAPVPVLPTPPEAPRPPATVHPEGVPPADSESKEVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF23 Antibody - BSA Free

Western Blot: PHF23 Antibody [NBP2-13756]

Western Blot: PHF23 Antibody [NBP2-13756]

Western Blot: PHF23 Antibody [NBP2-13756] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PHF23 Antibody [NBP2-13756]

Immunocytochemistry/ Immunofluorescence: PHF23 Antibody [NBP2-13756]

Immunocytochemistry/Immunofluorescence: PHF23 Antibody [NBP2-13756] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PHF23 Antibody [NBP2-13756]

Immunohistochemistry-Paraffin: PHF23 Antibody [NBP2-13756]

Immunohistochemistry-Paraffin: PHF23 Antibody [NBP2-13756] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Applications for PHF23 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHF23

PHD finger protein 23 (PHF23) contains 1 PHD (plant homeodomain)-type zinc finger and has been identified in acute myeloid leukemia as part of a fusion gene involving NUP98.

Alternate Names

FLJ16355, FLJ22884, hJUNE-1b, MGC2941, PDH-containing protein JUNE-1, PHD finger protein 23, PHD finger protein 23a

Gene Symbol

PHF23

Additional PHF23 Products

Product Documents for PHF23 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHF23 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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