PHKA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87281

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHKA1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHKA1 Antibody - BSA Free

Western Blot: PHKA1 Antibody [NBP1-87281]

Western Blot: PHKA1 Antibody [NBP1-87281]

Western Blot: PHKA1 Antibody [NBP1-87281] - Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PHKA1 Antibody [NBP1-87281]

Immunocytochemistry/ Immunofluorescence: PHKA1 Antibody [NBP1-87281]

Immunocytochemistry/Immunofluorescence: PHKA1 Antibody [NBP1-87281] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human small intestine shows low expression as expected.
Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining of human parathyroid gland shows high expression.
Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281]

Immunohistochemistry-Paraffin: PHKA1 Antibody [NBP1-87281] - Staining in human parathyroid gland and small intestine tissues using anti-PHKA1 antibody. Corresponding PHKA1 RNA-seq data are presented for the same tissues.

Applications for PHKA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHKA1

The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]

Alternate Names

MGC132604, PHKA, phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform, phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform, Phosphorylase kinase alpha M subunit, phosphorylase kinase, alpha 1 (muscle), phosphorylase kinase, alpha 1 (muscle), muscle glycogenosis

Gene Symbol

PHKA1

Additional PHKA1 Products

Product Documents for PHKA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PHKA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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