Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 338324
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human EGF R Y1068 site

Specificity

Detects human EGF R phosphorylated at Y1068 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Detection of EGF R/ErbB1 antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) was stained with Mouse Anti-Human Phospho-EGF R/ErbB1 (Y1068) Fluorescein-conjugated Monoclonal Antibody (Catalog # IC3570F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: EGFR

Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

For research use only

Citations for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

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