Recombinant Human EGF Protein, CF

(104 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is typically 20-100 pg/mL.
    The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
  • Source
    E. coli-derived Asn971-Arg1023, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    6 kDa
  • SDS-PAGE
    6 kDa, reducing conditions
236-EG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 20‑100 pg/mL.
Background: EGF
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
  • References:
    1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
    2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
    3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
    4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
    5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
    6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
    7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
    8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    HOMG4; URG; Urogastrone; beta-urogastrone; epidermal growth factor; epidermal growth factor (beta-urogastrone); HOMG4; pro-epidermal growth factor; URG
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 10 of 104
Filter your results:

Species
Applications
Sample Type
  1. Inhibition of PARP1 by small interfering RNA enhances docetaxel activity against human prostate cancer PC3 cells.
    Authors: Wu W, Kong Z, Duan X, Zhu H, Li S, Zeng S, Liang Y, Iliakis G, Gui Z, Yang D
    Biochem Biophys Res Commun, 2013;442(1):127-32.
    Species: Human
    Sample Type: whole cells
    Application: cell culture
  2. Oncogenic K-Ras and loss of Smad4 mediate invasion by activating an EGFR/NF-kappaB Axis that induces expression of MMP9 and uPA in human pancreas progenitor cells.
    Authors: Bera A, Zhao S, Cao L, Chiao P, Freeman J
    PLoS ONE, 2013;8(12):e82282.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  3. Diacylglycerol kinase delta modulates Akt phosphorylation through pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2).
    Authors: Crotty T, Nakano T, Stafforini D, Topham M
    J Biol Chem, 2013;288(3):1439-47.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  4. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013;4(0):e627.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  5. Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer.
    Authors: Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K
    Clin Cancer Res, 2012;18(22):6219-26.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  6. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012;7(9):e44477.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  7. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts.
    Authors: Goel S, Chin EN, Fakhraldeen SA
    J. Biol. Chem., 2012;287(20):16454-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
    Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
    J. Invest. Dermatol., 2010;130(5):1297-306.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer.
    Authors: Hickinson DM, Klinowska T, Speake G
    Clin. Cancer Res., 2010;16(4):1159-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Dithiolethione compounds inhibit Akt signaling in human breast and lung cancer cells by increasing PP2A activity.
    Authors: Switzer CH, Ridnour LA, Cheng RY, Sparatore A, Del Soldato P, Moody TW, Vitek MP, Roberts DD, Wink DA
    Oncogene, 2009;28(43):3837-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Docosahexaenoic acid promotes neuronal differentiation by regulating basic helix-loop-helix transcription factors and cell cycle in neural stem cells.
    Authors: Katakura M, Hashimoto M, Shahdat HM, Gamoh S, Okui T, Matsuzaki K, Shido O
    Neuroscience, 2009;160(3):651-60.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  13. Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase.
    Authors: Riento K, Frick M, Schafer I, Nichols BJ
    J. Cell. Sci., 2009;122(0):912-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
    Authors: Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J
    Cytotechnology, 2008;58(3):153-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo.
    Authors: Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D
    Clin. Cancer Res., 2008;14(9):2785-2795.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
    Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
    J. Biol. Chem., 2007;282(37):26656-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
    Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
    Biochem. Biophys. Res. Commun., 2007;363(3):472-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
    Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
    Biol. Reprod., 2007;77(1):53-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Effect of cytokine treatment on the neurogenesis process in the brain of soman-poisoned mice.
    Authors: Collombet JM, Four E, Burckhart MF, Masqueliez C, Bernabe D, Baubichon D, Herodin F, Lallement G
    Toxicology, 2005;210(1):9-23.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  20. Wa5 is a novel ENU-induced antimorphic allele of the epidermal growth factor receptor.
    Authors: Lee D, Cross SH, Strunk KE, Morgan JE, Bailey CL, Jackson IJ, Threadgill DW
    Mamm. Genome, 2004;15(7):525-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  21. Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina.
    Authors: Close JL, Liu J, Gumuscu B, Reh TA
    Glia, 2006;54(2):94-104.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  22. RGD-peptides for tissue engineering of articular cartilage.
    Authors: Jeschke B, Meyer J, Jonczyk A, Kessler H, Adamietz P, Meenen NM, Kantlehner M, Goepfert C, Nies B
    Biomaterials, 2002;23(16):3455-63.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  23. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
    Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
    Am. J. Kidney Dis., 2002;39(5):1078-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability.
    Authors: Chen MC, Goliger J, Bunnett N, Soll AH
    Am. J. Physiol. Gastrointest. Liver Physiol., 2001;280(2):G264-72.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  25. Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions.
    Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T
    Biochem. Biophys. Res. Commun., 2007;361(3):586-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Notch and Wnt signaling mediated rod photoreceptor regeneration by Muller cells in adult mammalian retina.
    Authors: Del Debbio CB, Balasubramanian S, Parameswaran S, Chaudhuri A, Qiu F, Ahmad I
    PLoS ONE, 2010;5(8):e12425.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
    Authors: Close JL, Gumuscu B, Reh TA
    Development, 2005;132(13):3015-26.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  28. Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
    Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
    Oncogene, 2010;29(20):2938-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003;23(13):5919-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  30. Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
    Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG
    J. Biol. Chem., 2008;283(46):31736-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
    Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
    Cancer Res., 1998;58(17):4008-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Insulin signaling via Akt2 switches plakophilin 1 function from stabilizing cell adhesion to promoting cell proliferation.
    Authors: Wolf A, Rietscher K, Glass M, Huttelmaier S, Schutkowski M, Ihling C, Sinz A, Wingenfeld A, Mun A, Hatzfeld M
    J Cell Sci, 2013;126(0):1832-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
    Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
    Dev. Biol., 2004;266(2):223-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  34. The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.
    Authors: Sa SM, Valdez PA, Wu J
    J. Immunol., 2007;178(4):2229-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. Mitogen-activated protein kinase-signaling regulates the ability of Muller glia to proliferate and protect retinal neurons against excitotoxicity.
    Authors: Fischer AJ, Scott MA, Ritchey ER, Sherwood P
    Glia, 2009;57(14):1538-52.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  36. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  37. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008;22(2):152-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  38. The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer.
    Authors: Mimura, Kousaku, Shiraishi, Kensuke, Mueller, Anja, Izawa, Shinichi, Kua, Ley-Fang, So, Jimmy, Yong, Wei-Peng, Fujii, Hideki, Seliger, Barbara, Kiessling, Rolf, Kono, Koji
    J Immunol, 2013;191(12):6261-72.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  39. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012;30(7):1455-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  40. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
    Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
    J. Immunol., 2002;169(5):2653-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. Human bronchial epithelial cells express and secrete MMP-12.
    Authors: Lavigne MC, Thakker P, Gunn J, Miyashiro JS, Wasserman AM, Wei SQ, Pelker JW
    Biochem. Biophys. Res. Commun., 2004;324(2):534-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads.
    Authors: Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI
    Am. J. Respir. Cell Mol. Biol., 2004;30(6):816-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. Regulation of IL-33 expression by IFN-gamma and tumor necrosis factor-alpha in normal human epidermal keratinocytes.
    J. Invest. Dermatol., 2012;132(11):2593-600.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  44. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006;10(1):39-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  45. Purification and characterization of mouse lacrimal gland epithelial cells and reconstruction of an acinarlike structure in three-dimensional culture.
    Authors: Ueda Y, Karasawa Y, Satoh Y, Nishikawa S, Imaki J, Ito M
    Invest. Ophthalmol. Vis. Sci., 2009;50(5):1978-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  46. Atrial-radiofrequency catheter ablation mediated targeting of mesenchymal stromal cells.
    Authors: Schweizer PA, Krause U, Becker R, Seckinger A, Bauer A, Hardt C, Eckstein V, Ho AD, Koenen M, Katus HA, Zehelein J
    Stem Cells, 2007;25(6):1546-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  47. Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
    Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
    Stem Cells, 2008;26(7):1705-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  48. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  49. EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
    Authors: Li JJ, Liu DP, Liu GT, Xie D
    Oncogene, 2009;28(15):1759-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  50. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
    Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
    Development, 2006;133(4):751-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  51. Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
    Authors: Lee HS, Myers A, Kim J
    Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  52. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  53. Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
    Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
    Genes Dev, 2014;28(10):1085-100.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  54. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
    Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
    Stroke, 2006;37(9):2361-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  55. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
    Authors: Spence JR, Mayhew CN, Rankin SA
    Nature, 2011;470(7332):105-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  56. Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
    Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
    J Exp Med, 2013;210(13):2851-72.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  57. CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
    Authors: Walter D, Satheesha S, Albrecht P, Bornhauser BC, D'Alessandro V, Oesch SM, Rehrauer H, Leuschner I, Koscielniak E, Gengler C, Moch H, Bernasconi M, Niggli FK, Schafer BW
    PLoS ONE, 2011;6(5):e19506.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  58. Targeting protein kinase CK2 suppresses prosurvival signaling pathways and growth of glioblastoma.
    Authors: Zheng Y, McFarland B, Drygin D, Yu H, Bellis S, Kim H, Bredel M, Benveniste E
    Clin Cancer Res, 2013;19(23):6484-94.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  59. The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.
    Authors: Park JA, Drazen JM, Tschumperlin DJ
    J. Biol. Chem., 2010;285(39):29817-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  60. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  61. Enhanced radiosensitivity and radiation-induced apoptosis in glioma CD133-positive cells by knockdown of SirT1 expression.
    Authors: Chang CJ, Hsu CC, Yung MC, Chen KY, Tzao C, Wu WF, Chou HY, Lee YY, Lu KH, Chiou SH, Ma HI
    Biochem. Biophys. Res. Commun., 2009;380(2):236-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  62. Multiple receptor tyrosine kinases converge on microRNA-134 to control KRAS, STAT5B, and glioblastoma.
    Authors: Zhang, Y, Kim, J, Mueller, A C, Dey, B, Yang, Y, Lee, D-h, Hachmann, J, Finderle, S, Park, D M, Christensen, J, Schiff, D, Purow, B, Dutta, A, Abounader, R
    Cell Death Differ, 2014;21(5):720-34.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  63. Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage.
    Authors: Fischer AJ, Omar G, Eubanks J, McGuire CR, Dierks BD, Reh TA
    Mol. Vis., 2004;10(0):973-86.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  64. Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
    Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
    Mol. Cancer Ther., 2008;7(10):3223-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  65. Vandetanib inhibits both VEGFR-2 and EGFR signalling at clinically relevant drug levels in preclinical models of human cancer.
    Authors: Brave SR, Odedra R, James NH
    Int. J. Oncol., 2011;39(1):271-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  66. Thalidomide inhibits epidermal growth factor-induced cell growth in mouse and human monocytic leukemia cells via Ras inactivation.
    Authors: Noman AS, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T
    Biochem. Biophys. Res. Commun., 2008;374(4):683-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  67. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  68. Bmi1 is essential in Twist1-induced epithelial-mesenchymal transition.
    Authors: Yang MH, Hsu DS, Wang HW
    Nat. Cell Biol., 2010;12(10):982-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  69. Dual roles of IL-4 in lung injury and fibrosis.
    Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
    J. Immunol., 2003;170(4):2083-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  70. Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration.
    Authors: Watanabe K, Bianco C, Strizzi L, Hamada S, Mancino M, Bailly V, Mo W, Wen D, Miatkowski K, Gonzales M, Sanicola M, Seno M, Salomon DS
    J. Biol. Chem., 2007;282(43):31643-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  71. Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function.
    Authors: Leslie J, Huang S, Opp J, Nagy M, Kobayashi M, Young V, Spence J
    Infect Immun, 2015;83(1):138-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  72. Upregulated WDR5 promotes proliferation, self-renewal and chemoresistance in bladder cancer via mediating H3K4 trimethylation.
    Authors: Chen X, Xie W, Gu P, Cai Q, Wang B, Xie Y, Dong W, He W, Zhong G, Lin T, Huang J
    Sci Rep, 2015;5(0):8293.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  73. Selective calcium sensitivity in immature glioma cancer stem cells.
    Authors: Wee S, Niklasson M, Marinescu V, Segerman A, Schmidt L, Hermansson A, Dirks P, Forsberg-Nilsson K, Westermark B, Uhrbom L, Linnarsson S, Nelander S, Andang M
    PLoS ONE, 2014;9(12):e115698.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  74. High levels of KAP1 expression are associated with aggressive clinical features in ovarian cancer.
    Authors: Cui Y, Yang S, Fu X, Feng J, Xu S, Ying G
    Int J Mol Sci, 2015;16(1):363-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  75. The evidence of glioblastoma heterogeneity.
    Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
    Sci Rep, 2015;5(0):7979.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  76. Activin/nodal signaling switches the terminal fate of human embryonic stem cell-derived trophoblasts.
    Authors: Sarkar P, Randall S, Collier T, Nero A, Russell T, Muddiman D, Rao B
    J Biol Chem, 2015;290(14):8834-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  77. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015;6(0):6576.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  78. Early intervention for spinal cord injury with human induced pluripotent stem cells oligodendrocyte progenitors.
    Authors: All A, Gharibani P, Gupta S, Bazley F, Pashai N, Chou B, Shah S, Resar L, Cheng L, Gearhart J, Kerr C
    PLoS ONE, 2015;10(1):e0116933.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  79. Acetylation of the RhoA GEF Net1A controls its subcellular localization and activity.
    Authors: Song E, Oh W, Ulu A, Carr H, Zuo Y, Frost J
    J Cell Sci, 2015;128(5):913-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  80. Epigenetic regulation of Elf5 is associated with epithelial-mesenchymal transition in urothelial cancer.
    Authors: Wu, Bo, Cao, Xiaoming, Liang, Xuezhi, Zhang, Xuhui, Zhang, Wei, Sun, Guang, Wang, Dongwen
    PLoS ONE, 2015;10(1):e0117510.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  81. Transcriptional profiling of adult neural stem-like cells from the human brain.
    Authors: Sandberg C, Vik-Mo E, Behnan J, Helseth E, Langmoen I
    PLoS ONE, 2014;9(12):e114739.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  82. mTOR-dependent proliferation defect in human ES-derived neural stem cells affected by myotonic dystrophy type 1.
    Authors: Denis J, Gauthier M, Rachdi L, Aubert S, Giraud-Triboult K, Poydenot P, Benchoua A, Champon B, Maury Y, Baldeschi C, Scharfmann R, Pietu G, Peschanski M, Martinat C
    J Cell Sci, 2013;126(0):1763-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  83. TGF-beta1 promotes lymphangiogenesis during peritoneal fibrosis.
    Authors: Kinashi H, Ito Y, Mizuno M, Suzuki Y, Terabayashi T, Nagura F, Hattori R, Matsukawa Y, Mizuno T, Noda Y, Nishimura H, Nishio R, Maruyama S, Imai E, Matsuo S, Takei Y
    J Am Soc Nephrol, 2013;24(10):1627-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  84. Distinct effects of ligand-induced PDGFRalpha and PDGFRbeta signaling in the human rhabdomyosarcoma tumor cell and stroma cell compartments.
    Authors: Ehnman M, Missiaglia E, Folestad E, Selfe J, Strell C, Thway K, Brodin B, Pietras K, Shipley J, Ostman A, Eriksson U
    Cancer Res, 2013;73(7):2139-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  85. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
    Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
    Int J Cancer, 2014;134(10):2489-503.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  86. Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model.
    Authors: Chen Y, Huang W, Chang S, Chang K, Kao S, Lo J, Su P
    Oncol Rep, 2013;30(6):2829-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  87. Treatment of acute respiratory distress syndrome with allogeneic adipose-derived mesenchymal stem cells: a randomized, placebo-controlled pilot study.
    Authors: Zheng G, Huang L, Tong H, Shu Q, Hu Y, Ge M, Deng K, Zhang L, Zou B, Cheng B, Xu J
    Respir Res, 2014;15(0):39.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  88. Sulforaphane, quercetin and catechins complement each other in elimination of advanced pancreatic cancer by miR-let-7 induction and K-ras inhibition.
    Authors: Appari M, Babu K, Kaczorowski A, Gross W, Herr I
    Int J Oncol, 2014;45(4):1391-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  89. Sulforaphane and TRAIL induce a synergistic elimination of advanced prostate cancer stem-like cells.
    Authors: Labsch S, Liu L, Bauer N, Zhang Y, Aleksandrowicz E, Gladkich J, Schonsiegel F, Herr I
    Int J Oncol, 2014;44(5):1470-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  90. The broad-spectrum metalloproteinase inhibitor BB-94 inhibits growth, HER3 and Erk activation in fulvestrant-resistant breast cancer cell lines.
    Authors: Kirkegaard T, Yde C, Kveiborg M, Lykkesfeldt A
    Int J Oncol, 2014;45(1):393-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  91. Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
    Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
    Int J Oncol, 2014;44(4):1118-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  92. Additive effects of EGF and IL-1beta regulate tumor cell migration and invasion in gastric adenocarcinoma via activation of ERK1/2.
    Authors: Han J, Xie Y, Lan F, Yu Y, Liu W, Chen J, Zheng F, Ouyang X, Lin X, Lin Y, Huang Q, Wang L, Tan J
    Int J Oncol, 2014;45(1):291-301.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  93. Endothelial-mesenchymal transition of brain endothelial cells: possible role during metastatic extravasation.
    Authors: Krizbai I, Gasparics A, Nagyoszi P, Fazakas C, Molnar J, Wilhelm I, Bencs R, Rosivall L, Sebe A
    PLoS ONE, 2015;10(3):e0119655.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  94. Mitochondrial control by DRP1 in brain tumor initiating cells.
    Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
    Nat Neurosci, 2015;18(4):501-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  95. alpha-Taxilin interacts with sorting nexin 4 and participates in the recycling pathway of transferrin receptor.
    Authors: Sakane H, Horii Y, Nogami S, Kawano Y, Kaneko-Kawano T, Shirataki H
    PLoS ONE, 0;9(4):e93509.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  96. EGF potentiation of VEGF production is cell density dependent in H292 EGFR wild type NSCLC cell line.
    Authors: Ranayhossaini D, Lu J, Mabus J, Gervais A, Lingham R, Fursov N
    Int J Mol Sci, 2014;15(10):17686-704.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  97. Rho GTPases RhoA and Rac1 mediate effects of dietary folate on metastatic potential of A549 cancer cells through the control of cofilin phosphorylation.
    Authors: Oleinik N, Helke K, Kistner-Griffin E, Krupenko N, Krupenko S
    J Biol Chem, 2014;289(38):26383-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  98. Chromatinized protein kinase C-theta directly regulates inducible genes in epithelial to mesenchymal transition and breast cancer stem cells.
    Authors: Zafar A, Wu F, Hardy K, Li J, Tu W, McCuaig R, Harris J, Khanna K, Attema J, Gregory P, Goodall G, Harrington K, Dahlstrom J, Boulding T, Madden R, Tan A, Milburn P, Rao S
    Mol Cell Biol, 2014;34(16):2961-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  99. Let-7 Sensitizes KRAS Mutant Tumor Cells to Chemotherapy.
    Authors: Dai X, Jiang Y, Tan C
    PLoS ONE, 2015;10(5):e0126653.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  100. Arf6 regulates EGF-induced internalization of E-cadherin in breast cancer cells.
    Authors: Xu R, Zhang Y, Gu L, Zheng J, Cui J, Dong J, Du J
    Cancer Cell Int, 2015;15(1):11.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  101. Characterisation of mesothelioma-initiating cells and their susceptibility to anti-cancer agents.
    Authors: Pasdar E, Smits M, Stapelberg M, Bajzikova M, Stantic M, Goodwin J, Yan B, Stursa J, Kovarova J, Sachaphibulkij K, Bezawork-Geleta A, Sobol M, Filimonenko A, Tomasetti M, Zobalova R, Hozak P, Dong L, Neuzil J
    PLoS ONE, 2015;10(5):e0119549.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  102. Mutation of androgen receptor N-terminal phosphorylation site Tyr-267 leads to inhibition of nuclear translocation and DNA binding.
    Authors: Karaca M, Liu Y, Zhang Z, De Silva D, Parker J, Earp H, Whang Y
    PLoS ONE, 2015;10(5):e0126270.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  103. Vaccination for invasive canine meningioma induces in situ production of antibodies capable of antibody-dependent cell-mediated cytotoxicity.
    Authors: Andersen B, Pluhar G, Seiler C, Goulart M, SantaCruz K, Schutten M, Meints J, O'Sullivan M, Bentley R, Packer R, Thomovsky S, Chen A, Faissler D, Chen W, Hunt M, Olin M, Ohlfest J
    Cancer Res, 2013;73(10):2987-97.
    Species: Canine
    Sample Type: Whole Cells
    Application: Bioassay
  104. An in vitro cord formation assay identifies unique vascular phenotypes associated with angiogenic growth factors.
    Authors: Falcon B, Swearingen M, Gough W, Lee L, Foreman R, Uhlik M, Hanson J, Lee J, McClure D, Chintharlapalli S
    PLoS ONE, 2014;9(9):e106901.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all
Animal-Free Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human EGF, Animal-Free Protein

BA AFL236
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Order Details

    Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Customers Who Viewed
This Item Also Viewed

Feedback

Customer Information