Phospho-GABA-B R2 (S892) Antibody

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PPS073
Detection of Phospho-GABAB R2 (S892) by Western Blot
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Phospho-GABA-B R2 (S892) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Xenopus
Specificity
Human, mouse, rat, and Xenopus ~110 kDa GABAB Receptor, R2 subunit phosphorylated at S892
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S892 of GABAB R2
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Immunofluorescence
1:100 - 1:250 dilution
(Couve, et al. 2002)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Phospho-GABAB R2 (S892) by Western Blot View Larger

Detection of Phospho-GABAB R2 (S892) by Western Blot
Western blots of COS-7 cells transiently expressing mutant GABAB R2 (S892A) (lane 1), wild type GABAB R2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABAB R2 (S892) (A). In B, the membrane was pre-treated with lambda -PPase (1200 units for 30 minutes) before being exposed to the anti-GABAB Receptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-B R2

The GABA-B ( gamma -aminobutyric acid-type B) receptors (GABABR) are C family members of the GPCR superfamily. There are only two family members, GABARB1(a & b) and GABARB2, which form a nondisulfide-linked heterodimeric receptor in the native state. GABARB heterodimers are found both pre- and post-synaptically, where they modulate glutamate release presynaptically, and prolong neuronal hyperpolarization postsynaptically. Rat GABARB2 is a 110 kDa, 900 amino acid (aa), 7-transmembrane glycoprotein. It has an extended, 442 aa N-terminal extracellular region that contains the GABA binding site. Unlike most other GPCRs, PKA phosphorylation of the GABAB2 cytoplasmic tail does not promote GABAB2 internalization. To the contrary, phosphorylation of S892 promotes surface expression, while dephosphorylation promotes internalization. Across species (human to frog to mouse to rat), there is 88% aa identity in the 200 aa cytoplasmic tail, and 85% aa identity over the entire mature receptor.

References
  1. Kornau, H-C. (2006) Cell Tissue Res. 326:517.
  2. Huang, Z. (2006) Neuron 50:521.
  3. Fairfax, B.J. et al. (2004) J. Biol. Chem. 279:12565.
  4. White, J.H. et al. (1998) Nature 396:679.
  5. Kaupmann, K. et al. (1998) Nature 396:683.
Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 2
Entrez Gene IDs
9568 (Human)
Alternate Names
G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099

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