Detection of Phospho-GABAB R2 (S892) by Western Blot
Western blots of COS-7 cells transiently expressing mutant GABAB R2 (S892A) (lane 1), wild type GABAB R2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABAB R2 (S892) (A). In B, the membrane was pre-treated with lambda -PPase (1200 units for 30 minutes) before being exposed to the anti-GABAB Receptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-B R2
The GABA-B ( gamma -aminobutyric acid-type B) receptors (GABABR) are C family members of the GPCR superfamily. There are only two family members, GABARB1(a & b) and GABARB2, which form a nondisulfide-linked heterodimeric receptor in the native state. GABARB heterodimers are found both pre- and post-synaptically, where they modulate glutamate release presynaptically, and prolong neuronal hyperpolarization postsynaptically. Rat GABARB2 is a 110 kDa, 900 amino acid (aa), 7-transmembrane glycoprotein. It has an extended, 442 aa N-terminal extracellular region that contains the GABA binding site. Unlike most other GPCRs, PKA phosphorylation of the GABAB2 cytoplasmic tail does not promote GABAB2 internalization. To the contrary, phosphorylation of S892 promotes surface expression, while dephosphorylation promotes internalization. Across species (human to frog to mouse to rat), there is 88% aa identity in the 200 aa cytoplasmic tail, and 85% aa identity over the entire mature receptor.
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gamma-Aminobutyric Acid Type B Receptor Subunit 2
Entrez Gene IDs:
G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099
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