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Phospho-STAT2 (Y689) Fluorescein-conjugated Antibody

Catalog #: IC2890F
1 Citation Datasheet / COA / SDS

Discontinued Product

IC2890F has been discontinued.
View all STAT2 products.
Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry.
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Phospho-STAT2 (Y689) Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT2 when phosphorylated at Y689 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing the human STAT2 Y689 site
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT2 (Y689) antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line untreated (light orange filled histogram) or treated with Recombinant Human IFN-aA (11100-1) (dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2890F) or isotype control antibody (IC105F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT2

STAT2 (Signal Transducer and Activator of Transcription #2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with Type I ( alpha - and beta -) Interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name
Signal Transducer and Activator of Transcription 2
Entrez Gene IDs
6773 (Human); 20847 (Mouse)
Alternate Names
interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2

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Citation for Phospho-STAT2 (Y689) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction.
    Authors: Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ
    J. Immunol., 2010-08-30;185(7):4457-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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