Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.
Phospho-Tyrosine Antibody (179003) [Agarose]
R&D Systems | Catalog # AGM1676
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Multi-Species
Applications
Immunoprecipitation
Label
Agarose
Antibody Source
Monoclonal Mouse IgG1 Clone # 179003
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Product Specifications
Immunogen
KLH-coupled phospho-tyrosine synthetic peptide
Specificity
Detects endogenous proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using ligand- and pervanadate-treated cell lysates indicate that clone 179003 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. Does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phospho-Tyrosine Antibody (179003) [Agarose]
Immunoprecipitation of Human Phospho-tyrosine.
A431 human epithelial carcinoma cell line was untreated (-) or treated (+) with 200 mM pervanadate (PV) for 15 minutes. Phospho-tyrosine was immunoprecipitated from 500 µg of cell lysate following incubation with 10 µg Mouse Anti-Phospho-Tyrosine Agarose-conjugated Monoclonal Antibody (Catalog # AGM1676) for 16 hours at 2 - 8 ºC. Immunoprecipitated phospho-tyrosine was detected by Western blot using 1 µg Phospho-Tyrosine Biotinylated Monoclonal Antibody (left panel, Catalog # BAM1676) or Human EGF R/ErbB1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (right panel, Catalog # BAF231). View our recommended buffer recipes for immunoprecipitation.Applications for Phospho-Tyrosine Antibody (179003) [Agarose]
Application
Recommended Usage
Immunoprecipitation
10 µL/500 µg cell lysate
Sample: A431 human epithelial carcinoma cell line treated with pervanadate, see our available Western blot detection antibodies
Sample: A431 human epithelial carcinoma cell line treated with pervanadate, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Formulation
Phosphate-buffered saline (PBS) with preservative.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Calculators
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody (179003) [Agarose]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Phospho-Tyrosine Antibody (179003) [Agarose]
For research use only
Related Research Areas
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Protocols
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