Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

Catalog # Availability Size / Price Qty
APM1676
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody Summary

Specificity
Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
Source
Monoclonal Mouse IgG1 Clone # 179003
Formulation
Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.
Label
Alkaline Phosphatase

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
ELISA
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

ELISA

Western Blot Detection of Phospho-Tyrosine by Western Blot. View Larger

Detection of Phospho-Tyrosine by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names
2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; Tyrosine

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reconstitution Buffers

Reviews for Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

There are currently no reviews for this product. Be the first to review Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody and earn rewards!

Have you used Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review