Phospho-Tyrosine Alkaline Phosphatase-conjugated Antibody

    
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  • Specificity
    Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.
  • Source
    Monoclonal Mouse IgG1 Clone # 179003
  • Formulation
    Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.
  • Label
    Alkaline Phosphatase
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • ELISA
    Please refer to the PDF datasheet for specifications.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
ELISA
Detection of Phospho-Tyrosine by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Phospho-Tyrosine
Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.
  • Alternate Names:
    2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; Tyrosine
Related Research Areas

FAQs

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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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