Phospho-Tyrosine Antibody (179003) [AP]

R&D Systems | Catalog # APM1676

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Multi-Species

Applications

Western Blot, ELISA

Label

Alkaline Phosphatase

Antibody Source

Monoclonal Mouse IgG1 Clone # 179003
View all Phospho-Tyrosine Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled Phospho-Tyrosine synthetic peptide

Specificity

Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Phospho-Tyrosine Antibody (179003) [AP]

Phospho-PDGF R beta ELISA Using APM1676 as Detection Antibody.

Detection of Phospho-Tyrosine antibody by Western Blot.

Detection of Phospho-Tyrosine by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Applications for Phospho-Tyrosine Antibody (179003) [AP]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

1:1000 dilution
Sample: Pervanadate-treated A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers and preservatives.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (179003) [AP]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-Tyrosine Antibody (179003) [AP]

For research use only

Related Research Areas

GDNF Family

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