Phospho-Tyrosine Antibody

(1 citations)
(1 Review)
  
  • Specificity
    Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence.
    No cross‑reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 179003
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-coupled phospho-tyrosine synthetic peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-Tyrosine by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.
Detection of Human Phospho-Tyrosine by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 15 minutes, loaded at 0.2 mg/mL. Tyrosine-phosphorylated proteins were detected (as indicated) using 50 µg/mL of Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunocytochemistry
Phospho-Tyrosine in A431 Human Cell Line. Phospho-Tyrosine was detected in immersion fixed A431 human epithelial carcinoma cell line treated with 50 ng/mL Recombinant Human EGF (left panel; Catalog # 236-EG) or untreated (right panel) using Mouse Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB1676) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Phospho-Tyrosine
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal cell cycle progression and oncogenic transformation. The role  of tyrosine phosphorylation is a matter of active and ongoing research. Specific antibodies that  recognize phosphorylated tyrosine residues are valuable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they are involved.
  • Alternate Names:
    2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; Tyrosine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Des-gamma-carboxyl prothrombin-promoted vascular endothelial cell proliferation and migration.
    Authors: Fujikawa T, Shiraha H, Ueda N, Takaoka N, Nakanishi Y, Matsuo N, Tanaka S, Nishina S, Suzuki M, Takaki A, Sakaguchi K, Shiratori Y
    J. Biol. Chem., 2007;282(12):8741-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Isotype Controls
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Mouse IgG1 Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Immunoprecipitation.

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Immunoprecipitation Phospho-Tyrosine Antibody MAB1676
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 IP Human Anonymous 06/28/2017
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Immunoprecipitation Phospho-Tyrosine Antibody MAB1676
Immunoprecipitation: Phospho-Tyrosine Antibody [MAB1676]

Summary

ApplicationImmunoprecipitation
Sample Tested293T human embryonic kidney cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsImmunoprecipitation with antibody for target protein and Western Blotting detection of phospholation.

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