Phospho-Tyrosine Antibody (RM111)
Novus Biologicals | Catalog # NBP2-77400
Recombinant Monoclonal Antibody
![Western Blot: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]](https://resources.rndsystems.com/images/products/Phosphoserine-Phosphothreonine-Phosphotyrosine-Antibody-RM111-Western-Blot-NBP2-77400-img0002.jpg "Western Blot: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
All Species
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM111 expressed in HEK293
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Product Specifications
Immunogen
Phospho-Tyrosine -BSA conjugate
Specificity
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phospho-Tyrosine Antibody (RM111)
![Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]](https://resources.rndsystems.com/images/products/Phosphoserine-Phosphothreonine-Phosphotyrosine-Antibody-RM111-Immunocytochemistry-Immunofluorescence-NBP2-77400-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]")
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]
Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using NBP2-77400 (followed by a PE conjugated secondary antibody, red) and DAPI (blue).![Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]](https://resources.rndsystems.com/images/products/Phosphoserine-Phosphothreonine-Phosphotyrosine-Antibody-RM111-Multiplex-Immunoassay-NBP2-77400-img0004.jpg "Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]")
Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]
Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Luminex analysis of NBP2-77400's reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. NBP2-77400 reacts only to phosphorylated Tyrosine.![Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]](https://resources.rndsystems.com/images/products/Phosphoserine-Phosphothreonine-Phosphotyrosine-Antibody-RM111-Immunoprecipitation-NBP2-77400-img0003.jpg "Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]")
Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]
Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Immunoprecipitation of EGF-treated A431 cells by NBP2-77400, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by NBP2-77400.Applications for Phospho-Tyrosine Antibody (RM111)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:200-1:1000
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:500
Immunohistochemistry
1:200 - 1:500
Immunoprecipitation
1:200-1:1000
Western Blot
1:1000 -1:5000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody (RM111)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Phospho-Tyrosine Antibody (RM111)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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