Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25981

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

All Species

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM111 expressed in HEK293

Format

Azide and BSA Free
View all Phospho-Tyrosine Primary Antibodies »

Product Specifications

Immunogen

Phospho-Tyrosine -BSA conjugate

Specificity

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -

Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunoprecipitation of EGF-treated A431 cells by Clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111.
Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -

Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Western blot of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.
Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).
Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -

Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 reacts only to phosphorylated Tyrosine.

Applications for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:200-1:1000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:500

Immunohistochemistry

1:200 - 1:500

Immunoprecipitation

1:200-1:1000

Western Blot

1:1000 -1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospho-Tyrosine

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

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Protocols

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FAQs

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