PKM2 Antibody (7B2) - BSA Free

Novus Biologicals | Catalog # NBP3-15230

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B2 expressed in HEK293

Format

BSA Free
View all PKM2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PKM2. UniProt ID (P14618).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKM2 Antibody (7B2) - BSA Free

Western Blot: PKM2 Antibody (7B2) [NBP3-15230]

Western Blot: PKM2 Antibody (7B2) [NBP3-15230]

Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230]

Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230]

Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230]

Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230]

Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230]

Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230]

Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230]

Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230]

Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).

Applications for PKM2 Antibody (7B2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PKM2

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Gene Symbol

PKM

Additional PKM2 Products

Product Documents for PKM2 Antibody (7B2) - BSA Free

Certificate of Analysis

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Product Specific Notices for PKM2 Antibody (7B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

HIF Enhancer Pathways HIF Enhancer Pathway Thumbnail