PLA2R1 Antibody (12-6-5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80912

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 12-6-5

Format

Azide and BSA Free
View all PLA2R1 Primary Antibodies »

Product Specifications

Immunogen

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3). Genbank acc no. U17033; amino acids 20-663 Recombinant protein expressed in HEK 293-EBNA-1 cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PLA2R1 Antibody (12-6-5) - Azide and BSA Free

Western Blot: PLA2R1 Antibody (12-6-5)Azide and BSA Free [NBP2-80912]

Western Blot: PLA2R1 Antibody (12-6-5)Azide and BSA Free [NBP2-80912]

Western Blot: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000. Image from the standard format of this antibody.
Immunocytochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunocytochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunocytochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - Normal human kidney showing glomerular specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection sy
Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - Normal human kidney showing podocyte specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection syst
Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - A surface stain was performed on HeLa cells with PLA2R1 Antibody (12-6-5) NBP2-50248PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.
Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens). Image from the standard format of this antibody.
Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dil
Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912]

Flow Cytometry: PLA2R1 Antibody (12-6-5) - Azide and BSA Free [NBP2-80912] - Overlay flow cytometry histograms of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) stained with Ms Cl12-6-5) at dilution 1:200. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line). Image from the standard format of this antibody.

Applications for PLA2R1 Antibody (12-6-5) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000 - 1:20000

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:5000 - 1:20000

Western Blot

1:5000 - 1:10000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (12-6-5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PLA2R1 Antibody (12-6-5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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