Plectin Antibody (7A8)

Novus Biologicals | Catalog # NB120-11220

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Mammal

Cited:

Bovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Dot Blot, Electron Microscopy

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7A8
View all Plectin Primary Antibodies »

Product Specifications

Immunogen

Plectin purified from rat glioma C6 cells.

Epitope

Monoclonal Anti-Plectin recognizes an epitope located in the middle section on the rod domain of the plectin molecule, approximately half way between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as thi

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30591979).Cross-reacts with Marsupials. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The inhibits the interaction of plectin with vimentin and lamin B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Plectin Antibody (7A8)

Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220]

Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220]

Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220] - Frozen sections acetone fixed Rat cardiac muscle tissue sections were stained with 1:200 Monoclonal Anti-Plectin antibody produced in Mouse, Clone: 7A8, followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.

Applications for Plectin Antibody (7A8)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
WB: Detects a band of approximately 300 kDa.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Plectin

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Alternate Names

EBS1, HD1, Hemidesmosomal protein 1, LGMD2Q, PCNplectin 1, intermediate filament binding protein, 500kD, PLEC1b, PLEC1EBSO, plectin, plectin 1, intermediate filament binding protein 500kDa, plectin-1, PLTNepidermolysis bullosa simplex 1 (Ogna)

Entrez Gene IDs

64204 (Rat)

Gene Symbol

PLEC

Additional Plectin Products

Product Documents for Plectin Antibody (7A8)

Certificate of Analysis

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Product Specific Notices for Plectin Antibody (7A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Plectin Antibody (7A8)

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Protocols

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