PLGF Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NB600-1012
![Western Blot: PLGF Antibody [NB600-1012]](https://beta-resources.rndsystems.com/images/products/PLGF-Antibody-Western-Blot-NB600-1012-img0002.jpg "Western Blot: PLGF Antibody [NB600-1012]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
N-terminal peptide (20aa) of native human PlGF
Localization
Cell Membrane and Secreted
Specificity
The will react with all human PLGF isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PLGF Antibody - Azide and BSA Free (NB600-1012) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PLGF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PLGF Antibody - Azide and BSA Free
Western Blot: PLGF Antibody [NB600-1012]
Western Blot: PLGF Antibody [NB600-1012] - Western Blot analysis of human PLGF isoforms with a polyclonal antibody directed against an N-terminal peptide of native human PLGF. There is a weak cross reactivity with rat PLGF.![Western Blot: PLGF Antibody [NB600-1012]](https://beta-resources.rndsystems.com/images/products/PlGF-Antibody-Western-Blot-NB600-1012-img0001.jpg "Western Blot: PLGF Antibody [NB600-1012]")
Western Blot: PLGF Antibody [NB600-1012]
Western Blot: PlGF Antibody [NB600-1012] - Analysis of human PlGF-2 with a polyclonal antibody directed against a N-terminal peptide of native human PlGF. There is a strong cross reactivity with mouse PlGF.Applications for PLGF Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5 ug/ml
Immunocytochemistry/ Immunofluorescence
5-25ug/ml
Western Blot
2-10 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: PlGF
Long Name
Placenta Growth Factor
Alternate Names
PGF, PGFL
Entrez Gene IDs
5228 (Human)
Gene Symbol
PGF
UniProt
Additional PlGF Products
Product Documents for PLGF Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PLGF Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for PLGF Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PLGF Antibody - Azide and BSA Free
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Q: Does the Human PlGF Quantikine® ELISA Kit, Catalog # DPG00, detect free or total PlGF?
A: Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested in the Quantikine® Immunoassay, the possibility of interference cannot be excluded. We cannot claim Catalog # DPG00 to be a "free" or "total" PlGF detecting kit, since all possible binding factors have not been tested. Specificity testing performed using rhFlt-1/Fc (VEGF R1) for interference demonstrated interference at levels greater than 2000 pg/mL. Details of this testing can be found in the DPG00 product insert. Most samples will have lower levels of sFlt-1 than 2000 pg/ml.
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