Pluripotent Stem Cell Transcription Factor Antibody Pack

Novus Biologicals | Catalog # NBP1-42823

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Kit Type

Kit

Product Summary for Pluripotent Stem Cell Transcription Factor Antibody Pack

This pack contains 1 vial of each: NB100-2379 (0.1 mL), NB110-37235 (0.1 mL), and NB100-58842 (0.1 mL).

Product Specifications

Clonality

Polyclonal

Specificity

NB100-58842 is specific for mouse Nanog protein. NB100-2379-OCT4 - Embryonic Stem Cell Marker NB110-37235 - SOX2 - Embryonic Stem Cell Marker

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Pluripotent Stem Cell Transcription Factor Antibody Pack

Immunocytochemistry/ Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Immunocytochemistry/ Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg
Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).
Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]
Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823]

Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Preservative

0.05% Sodium Azide

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: Pluripotent Stem Cell Transcription Factor

Pluripotent Stem Cell Transcription Factor antibody pack is a comprehensive research kit for measuring expression of the powerful stem cell regulators - Nanog, Oct3/4, and Sox2. All of the antibodies provided in this panel have been tested and are highly specific in flow cytometry application. They can be used simultaneously to analyze pluripotent stem cells in heterogenous stem cell populations.

Additional Pluripotent Stem Cell Transcription Factor Products

Product Documents for Pluripotent Stem Cell Transcription Factor Antibody Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pluripotent Stem Cell Transcription Factor Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Citations for Pluripotent Stem Cell Transcription Factor Antibody Pack

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 09/02/2015
    Sox2 and Oct4 expression in human breast cancer cell lines. NBP1-42823
    Pluripotent Stem Cell Transcription Factor Antibody Pack NBP1-42823

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Protocols

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