Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Sheep

Cited:

Human, Mouse, Rat, Canine

Predicted:

Chicken (92%). Backed by our 100% Guarantee.

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31690408).

Localization

Nuclear

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SOX2 Antibody - BSA Free (NB110-37235) is a polyclonal antibody validated for use in Multiplex Immunofluorescence, IHC, WB, Flow and ICC/IF. Anti-SOX2 Antibody: Cited in 37 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SOX2 Antibody - BSA Free

SOX2 Antibody - BSA Free Detection of SOX2 in Mouse Brain Cortex via seqIF™ staining on COMET™

Detection of SOX2 in Mouse Brain Cortex via seqIF™ staining on COMET™

SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Brain Cortex using Rabbit Anti-Mouse SOX2, Monoclonal Antibody (Catalog # NB110-37235) at 1:125 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder.

Immunohistochemistry-Paraffin: SOX2 Antibody - BSA Free [NB110-37235]

Immunohistochemistry-Paraffin: SOX2 Antibody - BSA Free [NB110-37235]

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.
Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.
Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.
Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235]

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 AntibodyBSA Free [NB110-37235]

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for SOX2 Antibody - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1:100

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50 - 1:250

Immunohistochemistry

1:125 - 1:250

Immunohistochemistry-Paraffin

1:125 - 1:250

Multiplex Immunofluorescence

1:125

Western Blot

0.5 ug/mL
Application Notes
This SOX2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot analysis, where a band is observed at ~40 kDa representing the SOX2 protein.

Reviewed Applications

Read 4 reviews rated 4.3 using NB110-37235 in the following applications:

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOX2

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Entrez Gene IDs

6657 (Human); 20674 (Mouse)

Gene Symbol

SOX2

UniProt

P48431

Additional SOX2 Products

Product Documents for SOX2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOX2 Antibody - BSA Free

Customer Reviews for SOX2 Antibody - BSA Free (4)

4.3 out of 5
4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • SOX2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/18/2020
    MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed.
    SOX2 Antibody - BSA Free NB110-37235
  • SOX2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human head and neck cancer cell lines
    Species: Human
    Verified Customer | Posted 10/11/2018
    The expression of pluripotency factors Nanog, Sox2, and Oct4 in human HNSCC cell lines FaDu and HN5.
    SOX2 Antibody - BSA Free NB110-37235
  • SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: aptient derived adgerent glioblastoma stem cells and patient-derived glioblastoma stem cells (adherent on laminin coating)
    Species: Human
    Verified Customer | Posted 04/24/2017
    dilution 1:250 in antibody buffer (1% BSA, 0.1% triton X in PBS)
    SOX2 Antibody - BSA Free NB110-37235
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Oral cancer cell line
    Species: Human
    Verified Customer | Posted 12/27/2011

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Protocols

View specific protocols for SOX2 Antibody - BSA Free (NB110-37235):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer all the time).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SOX2 Antibody - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

    • Q: As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.
      A: This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For SOX2 Antibody NB110-37235, the exact immunogen is available to me in our internal notes. The UniProt Blast of this gave a 100% match with the (again, unfortunately unreviewed) horse entries F6PUQ2 and B6VA41. A 100% match indicates that the antibody should be suitable for you. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

    • A: For the validation of this antibody in IHC-P, we performed antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0. Please note that Domyan et al 2011 from University of Wisconsin-Madison (USA) have cited the use of this antibody in IHC-P application in their experiments published in - Development (2011) 138, 971-981 Biologists site.

    • Q: We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
      A: For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
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