PMCA4 Antibody (JA9)

Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569]

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.

Western Blot: PMCA4 Antibody (JA9) [NB300-569] -

MiR-4261 can target and regulate the ATP2B4 gene and reduce the expression of PMCA4 protein. (A) Schematic diagram of the predicted miR-4261 binding site in the 3’-UTR of ATP2B4 mRNA. (B) Dual-luciferase reporter assay. The 3’-UTR fragments (wild-type and mutant sequences) of ATP2B4 were cloned into the psiCHECK-2 vector and then cotransfected with miR-4261 mimics or NC. Comparison of relative firefly/Renilla luciferase activity between the wild-type (WT) and negative control (NC) groups (PWT+mimics vs. WT+NC = 1.9E-4, and PWT+mimics vs. MUT+mimics = 1.4E-3). (C) The content of miR-4261 in HEK293T cells 36, 48, and 72 hours after transfection of miR-4261 mimics (P = 2.7E-2) and the endogenous content of miR-4261 in H929 cells and RPMI8226 cells. (D) The mRNA level of ATP2B4 was reduced by miR-4261, and the relative expression decreased most significantly at 72 hours after transfection (GAPDH was used as an internal control) (P = 3.6E-2). (E, F) MiR-4261 reduced the protein level of ATP2B4 (GAPDH was used as an internal control) (P = 4.7E-2). (Error bars represent the mean +/- SEM, *P < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36091107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PMCA4 Antibody (JA9) [NB300-569] -

Reduced expression of PMCA4 in RBCs of MM. (A, B) Indirect cellular immunofluorescence labeling of the surface of RBCs with PMCA4 (blue), RBC membrane with Did labeling (red), PMCA4 expression in MM RBCs is significantly reduced (P = 5.1E-03). The white arrow indicates the stacking of RBCs (rouleaux formation) in MM (magnification, 60×). (C) Relative mRNA expression of ATPase plasma membrane Ca2+ transport 4 (ATP2B4) in nucleated RBCs of MM bone marrow is significantly lower than that in the normal control group (GAPDH was used as an internal control) (P = 5.1E-03). (D, E) The expression of PMCA4 in MM RBCs was significantly reduced (P = 5.1E-03), and CD138 was expressed. (F) After the antibody-labeled RBCs reacted with the substrate BCIP/NBT, the dark blue NBT-formazan deposits in the erythrocyte membrane of MM reacting with PMCA4 protein content were significantly lower than those of the normal control group. (Error bars represent the mean +/- SEM, *P < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36091107), licensed under a CC-BY license. Not internally tested by Novus Biologicals.