PNPO Antibody (2C7) - BSA Free

Novus Biologicals | Catalog # NBP1-97941

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C7

Format

BSA Free
View all PNPO Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PNPO (57-261aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PNPO Antibody (2C7) - BSA Free

Western Blot: PNPO Antibody (2C7)BSA Free [NBP1-97941]

Western Blot: PNPO Antibody (2C7)BSA Free [NBP1-97941]

Western Blot: PNPO Antibody (2C7) [NBP1-97941] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanPNPO antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: A549 cell lysateLane 2.: HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunocytochemistry/ Immunofluorescence: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunocytochemistry/Immunofluorescence: PNPO Antibody (2C7) [NBP1-97941] - Analysis of PNPO in Hep3B cells. The cell was stainedwith ATGA0213 (1:100). The secondary antibody (green) wasused Alexa Fluor 488. DAPI was stained the cell nucleus(blue).
Immunohistochemistry-Paraffin: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunohistochemistry-Paraffin: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunohistochemistry-Paraffin: PNPO Antibody (2C7) [NBP1-97941] - Paraffin embedded sections of human breast cancertissue were incubated with anti-human PNPO (1:150) for2 hours at room temperature. Antigen retrieval wasperformed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine(DAB).
Flow Cytometry: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Flow Cytometry: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Flow Cytometry: PNPO Antibody (2C7) [NBP1-97941] - Flow cytometry analysis of PNPO in Jurkat cells. The cell wasstained at 2-5ug for 1x106cells (red). A Goatanti mouse IgG (Alexa fluor 488) was used as the secondaryantibody. Mouse monoclonal IgG was used as the isotypecontrol (dark gray), cells without incubation with primary andsecondary antibody was used as the negative control (lightgray).

Applications for PNPO Antibody (2C7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNPO

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Alternate Names

EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase

Gene Symbol

PNPO

UniProt

NP_060599 (Human)

Additional PNPO Products

Product Documents for PNPO Antibody (2C7) - BSA Free

Certificate of Analysis

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Product Specific Notices for PNPO Antibody (2C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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