POLR3A Antibody (HL3437)

Novus Biologicals | Catalog # NBP3-48813

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3437
View all POLR3A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human POLR3A.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3A Antibody (HL3437)

POLR3A Antibody (HL3437)

Western Blot: POLR3A Antibody (HL3437) [NBP3-48813] -

Whole zebrafish extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
POLR3A Antibody (HL3437)

Immunohistochemistry-Paraffin: POLR3A Antibody (HL3437) [NBP3-48813] -

POLR3A antibody [HL3437] detects POLR3A protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
POLR3A stained by POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.
POLR3A Antibody (HL3437)

Western Blot: POLR3A Antibody (HL3437) [NBP3-48813] -

Whole Japanese medaka extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
POLR3A Antibody (HL3437)

Immunohistochemistry-Paraffin: POLR3A Antibody (HL3437) [NBP3-48813] -

POLR3A antibody [HL3437] detects POLR3A protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
POLR3A stained by POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.
POLR3A Antibody (HL3437)

Immunocytochemistry/ Immunofluorescence: POLR3A Antibody (HL3437) [NBP3-48813] -

POLR3A antibody [HL3437] detects POLR3A protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: POLR3A stained by POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
POLR3A Antibody (HL3437)

Western Blot: POLR3A Antibody (HL3437) [NBP3-48813] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
POLR3A Antibody (HL3437)

Immunohistochemistry-Paraffin: POLR3A Antibody (HL3437) [NBP3-48813] -

POLR3A antibody [HL3437] detects POLR3A protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
POLR3A stained by POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
POLR3A Antibody (HL3437)

Western Blot: POLR3A Antibody (HL3437) [NBP3-48813] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLR3A antibody [HL3437] (NBP3-48813) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for POLR3A Antibody (HL3437)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3A

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Alternate Names

DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, DNA-directed RNA polymerase III subunit RPC1, EC 2.7.7.6, hRPC155, POL3A, polymerase (RNA) III (DNA directed) polypeptide A, 155kDa, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C160, RNA polymerase III subunit RPC155-D, RPC1, RPC155RNA polymerase III subunit C1

Gene Symbol

POLR3A

Additional POLR3A Products

Product Documents for POLR3A Antibody (HL3437)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for POLR3A Antibody (HL3437)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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