Porcine CD31/PECAM-1 Antibody Summary
Accession # Q95242
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD31/PECAM‑1 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells were stained with Rat Anti-Porcine CD31/PECAM-1 Monoclonal Antibody (Catalog # MAB33871, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD31, also known as PECAM-1 (platelet-endothelial cell adhesion molecule-1), is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to the vascular system, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1‑3). The extracellular domain (ECD) of CD31 has ten potential N-glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue‑ and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Porcine CD31 ECD shows 74%, 73%, 70%, 63% and 62% amino acid (aa) identity with bovine, canine, human, mouse and rat CD31, respectively. CD31 participates with other adhesion molecules for most functions but is the critical molecule for TEM. Homotypic CD31 adhesion in trans combined with cycling of CD31 to and from surface-connected endothelial cell vesicles leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31-/- mice are deficient in chemokine-mediated chemotaxis (14).
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Citations for Porcine CD31/PECAM-1 Antibody
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Citations: Showing 1 - 4
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In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds
Authors: M Albanna, KW Binder, SV Murphy, J Kim, SA Qasem, W Zhao, J Tan, IB El-Amin, DD Dice, J Marco, J Green, T Xu, A Skardal, JH Holmes, JD Jackson, A Atala, JJ Yoo
Sci Rep, 2019;9(1):1856.
Sample Types: Whole Tissue
3D artificial round section micro-vessels to investigate endothelial cells under physiological flow conditions
Authors: R Sfriso, S Zhang, CA Bichsel, O Steck, A Despont, OT Guenat, R Rieben
Sci Rep, 2018;8(1):5898.
Sample Types: Whole Cells
Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood
Authors: AK Bongoni, E Salvaris, S Nordling, N Klymiuk, E Wolf, DL Ayares, R Rieben, PU Magnusson, PJ Cowan
Sci Rep, 2017;7(1):4450.
Sample Types: Whole Cells
Effect of C1-INH on ischemia/reperfusion injury in a porcine limb ex vivo perfusion model
Authors: MM Abdelhafez, J Shaw, D Sutter, J Schnider, Y Banz, H Jenni, E Voegelin, MA Constantin, R Rieben
Mol. Immunol., 2017;88(0):116-124.
Sample Types: Whole Tissue
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