Porcine IFN-gamma Quantikine ELISA Kit

R&D Systems | Catalog # PIF00

R&D Systems
Discontinued Product
PIF00 has been discontinued. An alternative/replacement product is available: DY985. View all IFN-gamma products.

Key Product Details

Assay Length

4 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

11.2 pg/mL

Assay Range

39-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all IFN-gamma ELISA Kits »

Product Summary for Porcine IFN-gamma Quantikine ELISA Kit

The Quantikine Porcine IFN-gamma Immunoassay is a 4 hour solid-phase ELISA designed to measure porcine IFN-gamma in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring porcine IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IFN-gamma.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Porcine

Specificity

Natural and recombinant porcine IFN-gamma

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 29 34 34
Mean (pg/mL) 124 272 913 120 282 904
Standard Deviation 5.8 7.4 26.5 14.0 15.7 84.7
CV% 4.7 2.7 2.9 11.7 5.6 9.4

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 36 32 36
Mean (pg/mL) 226 449 1518 227 515 1522
Standard Deviation 10.1 12.0 40.4 17.1 51.3 97.7
CV% 4.5 2.7 2.7 7.5 10.0 6.4

Recovery for Porcine IFN-gamma Quantikine ELISA Kit

The recovery of porcine IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 97 84-116
EDTA Plasma (n=5) 98 85-111
Heparin Plasma (n=5) 106 86-118
Serum (n=5) 98 81-119

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of porcine IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Porcine IFN-gamma ELISA Linearity

Scientific Data Images for Porcine IFN-gamma Quantikine ELISA Kit

Porcine IFN-gamma ELISA Calibrator Diluent RD5T Standard Curve

Porcine IFN-gamma ELISA Calibrator Diluent RD5T Standard Curve

Porcine IFN-gamma ELISA Calibrator Diluent RD6-3 Standard Curve

Porcine IFN-gamma ELISA Calibrator Diluent RD6-3 Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for Porcine IFN-gamma Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Porcine IFN-gamma Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Porcine IFN-gamma Quantikine ELISA Kit

Customer Reviews for Porcine IFN-gamma Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Porcine IFN-gamma Quantikine ELISA Kit and earn rewards!

Have you used Porcine IFN-gamma Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Porcine IFN-gamma Quantikine ELISA Kit (PIF00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
  7.   Aspirate and wash 5 times.
    8. 200 µL Streptavidin-HRP
  8.   Add 200 µL Streptavidin-HRP to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes on the shaker.
  9.   Aspirate and wash 5 times.
    10. 120 µL Substrate Solution
  10. Add 120 µL Substrate Solution to each well.
  11. For Serum & Plasma Samples: Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.
    12. 120 µL Stop Solution
  12. Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits