POU3F3 Antibody

Novus Biologicals | Catalog # NBP1-49872

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Predicted:

Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all POU3F3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HMLSHAHQWVTAL corresponding to internal region according to NP_006227.1.

Epitope

HMLSHAHQWVTAL - internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for POU3F3 Antibody

Western Blot: POU3F3 Antibody [NBP1-49872]

Western Blot: POU3F3 Antibody [NBP1-49872]

Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872]

Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP1-49872]

Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: POU3F3 Antibody [NBP1-49872]

Flow Cytometry: POU3F3 Antibody [NBP1-49872]

Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for POU3F3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/mL
Application Notes
IHC-Fr reactivity reported in (PMID: 25273085). IHC reactivity reported in (PMID: 25224226).

Reviewed Applications

Read 1 review rated 5 using NBP1-49872 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POU3F3

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences Sumiyama et al. (1996) [PubMed 8703082].[supplied by OMIM]

Alternate Names

Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3

Entrez Gene IDs

5455 (Human); 18993 (Mouse); 192109 (Rat)

Gene Symbol

POU3F3

UniProt

NP_006227.1 (Human)

Additional POU3F3 Products

Product Documents for POU3F3 Antibody

Certificate of Analysis

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Product Specific Notices for POU3F3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POU3F3 Antibody

Customer Reviews for POU3F3 Antibody (1)

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Customer Images

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  • Name: Woo-Yang Kim
    Application: Immunofluorescence
    Sample Tested: Mouse Brain Tissue
    Species: Mouse
    Verified Customer | Posted 01/12/2015
    POU3F3
    POU3F3 Antibody NBP1-49872

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