Catalog Number: 0625
Chemical Name: Pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid tetrasodium salt
Biological Activity
A non-selective P2 purinergic antagonist. Blocks recombinant P2X1, P2X2, P2X3, P2X5 (IC50 = 1 - 2.6 μM), native P2Y2-like (IC50 ~ 0.9 mM), and recombinant P2Y4 (IC50 ~ 15 mM) receptors. Delays onset of calcium responses to mild hypoosmotic stress in cortical slices.

2'5'-isomer also available.

Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in water
  • Storage:
    Desiccate at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Other Product-Specific Information:
Background References
  1. Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema.
    Thrane et al.
    Proc.Natl.Acad.Sci.U S A, 2011;108:846
  2. Receptors for purines and pyrimidines.
    Ralevic and Burnstock
    Pharmacol.Rev., 1998;50:413
  3. Investigation of the actions of PPADS, a novel P2x-purinoceptor antagonist, in the guinea-pig isolated vas deferens.
    McLaren et al.
    Br.J.Pharmacol., 1994;111:913
  4. Selective antagonism by PPADS at P2x-purinoceptors in rabbit isolated blood vessels.
    Ziganshin et al.
    Br.J.Pharmacol., 1994;111:923
  5. PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses.
    Lambrecht et al.
    Eur.J.Pharmacol., 1992;217:217

The citations listed below are publications that use Tocris products. Selected citations for PPADS tetrasodium salt include:

17 Citations: Showing 1 - 10
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  1. Cortical layer 1 and layer 2/3 astrocytes exhibit distinct calcium dynamics in vivo.
    Authors: Takata and Hirase
    PLoS One ;3:e2525
  2. Duloxetine inhibits microglial P2X4 receptor function and alleviates neuropathic pain after peripheral nerve injury
    Authors: Yamashita Et al.
    PLoS One 2016;11:e0165189
  3. Intracellular calcium dynamics in cortical microglia responding to focal laser injury in the PC::G5-tdT reporter mouse.
    Authors: Pozner Et al.
    Front Mol Neurosci 2015;8:12
  4. Diadenosine tetraphosphate contributes to carbachol-induced tear secretion.
    Authors: Fonseca Et al.
    Purinergic Signal 2015;11:87
  5. Pharmacological and molecular characterization of functional P2 receptors in rat embryonic cardiomyocytes.
    Authors: Cheung Et al.
    J Mol Neurosci 2015;11:127
  6. NTPDase1 activity attenuates microglial phagocytosis.
    Authors: Bulavina Et al.
    Purinergic Signal 2013;9:199
  7. Blockade of P2 nucleotide receptors after spinal cord injury reduced the gliotic response and spared tissue.
    Authors: Rodríguez-Zayas Et al.
    Neuropsychopharmacology 2012;46:167
  8. The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.
    Authors: Kouzaki Et al.
    J Immunol 2011;186:4375
  9. Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema.
    Authors: Thrane Et al.
    Proc Natl Acad Sci U S A 2011;108:846
  10. An excitatory loop with astrocytes contributes to drive neurons to seizure threshold.
    Authors: Gómez-Gonzalo Et al.
    PLoS Biol 2010;8:e1000352
  11. Identification of atropine- and P2X1 receptor antagonist-resistant, neurogenic contractions of the urinary bladder.
    Authors: Kennedy Et al.
    J Neurosci 2007;27:845
  12. Multiple P2X receptors on guinea-pig pelvic ganglion neurons exhibit novel pharmacological properties.
    Authors: Zhong Et al.
    Br J Pharmacol 2001;132:221
  13. Rat chromaffin cells lack P2X receptors while those of the guinea-pig express a P2X receptor with novel pharmacology.
    Authors: Liu Et al.
    Br J Pharmacol 1999;128:61
  14. Evidence that the ATP-induced increase in vasomotion of guinea-pig mesenteric lymphatics involves an endothelium-dependent release of thromboxane A2.
    Authors: Gao Et al.
    Br J Pharmacol 1999;127:1597
  15. Pharmacological and molecular characterization of P2X receptors in rat pelvic ganglion neurons.
    Authors: Zhong Et al.
    Br J Pharmacol 1998;125:771
  16. P2X receptor-mediated excitation of nociceptive afferents in the normal and arthritic rat knee joint.
    Authors: Dowd Et al.
    Br J Pharmacol 1998;125:341
  17. Modulation of extracellular GABA levels in the retina by activation of glial P2X-purinoceptors.
    Authors: Neal Et al.
    Br J Pharmacol 1998;124:317
Expand to show all 17 Citations

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