PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

Novus Biologicals | Catalog # NB600-636

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Human, Mouse, Rat, Canine, Equine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPAR alpha/NR1C1 Primary Antibodies »

Product Specifications

Immunogen

PPAR alpha/NR1C1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha/NR1C1. (Uniprot: P23204)

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha/NR1C1 protein from chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha/NR1C1 homologues from other sources has not been determined
Human reactivity reported in scientific literature (PMID: 31095524).

Localization

Nuclear

Specificity

No reactivity is expected against other subtypes of PPAR.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is directed against mouse PPAR alpha/NR1C1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

Western Blot: PPAR alpha/NR1C1 AntibodyN-terminal [NB600-636]

Western Blot: PPAR alpha/NR1C1 AntibodyN-terminal [NB600-636]

Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: 50 kDa for PPAR Alpha.
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 ug/mL, 1h at RT degrees. Secondary antibody: Anti-RABBIT IgG DyLight (R) 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.
Immunohistochemistry: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

PPAR-alpha-NR1C1-Antibody---N-terminal-Immunohistochemistry-NB600-636-img0022.jpg
Western Blot: PPAR alpha/NR1C1 AntibodyN-terminal [NB600-636]

Western Blot: PPAR alpha/NR1C1 AntibodyN-terminal [NB600-636]

Western Blot: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions: Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ug/mL overnight at 4C. Secondary antibody: Alexa Fluor 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventricle (exit point of progenitor olfactory neurons); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636]

ELISA: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins.
PPAR alpha/NR1C1 Antibody - N-terminal

PPAR alpha/NR1C1 Antibody - N-terminal

Immunohistochemistry using anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
PPAR alpha/NR1C1 Antibody - N-terminal

PPAR alpha/NR1C1 Antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) showing PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
PPAR alpha/NR1C1 Antibody - N-terminal

PPAR alpha/NR1C1 Antibody - N-terminal

Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
PPAR alpha/NR1C1 Antibody - N-terminal

PPAR alpha/NR1C1 Antibody - N-terminal

Western Blot of Rabbit anti-PPAR Alpha (N-terminal specific) antibody. Marker: Opal Pre-stained ladder
PPAR alpha/NR1C1 Antibody - N-terminal

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] -

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody - N-terminal [NB600-636] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (a–f) & PPARalpha (g–i) immunolabeling. (a–c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells (a) which showed bright GPR55 immunolabelling (b). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d–f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) & glial fibrillary acidic protein (GFAP) immunoreactivity; open arrows indicate SGCs which were GPR55 immunoreactive & GFAP negative (e). Stars indicate sensory neurons of different dimension, which expressed faint –to-moderate GPR55 immunoreactivity. (g–i) White arrows indicate the Neurotrace labeled nuclei of SGCs which showed PPARalpha immunoreactivity (h). Open arrow indicate autofluorescent pigment. Bar: a–i = 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31608295), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

ELISA

1:75000-1:125000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1-5ug/ml

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500-1:2000
Application Notes
This product has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Use in chromatin immunoprecipitation reported in scientific literature (PMID 26586378).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing hiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPARgamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

19013 (Mouse); 25747 (Rat)

Gene Symbol

PPARA

UniProt

P23204 (Mouse)

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

Certificate of Analysis

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Product Specific Notices for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

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Protocols

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FAQs for PPAR alpha/NR1C1 Antibody - N-terminal - BSA Free

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  • Q: Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two

    A:

    Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

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