PPP2R1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-37951

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPP2R1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 468-667 of human PPP2R1B (NP_859050.1).

Sequence:
YAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPP2R1B Antibody - BSA Free

PPP2R1B Antibody

Immunocytochemistry/ Immunofluorescence: PPP2R1B Antibody [NBP3-37951] -

Immunocytochemistry/ Immunofluorescence: PPP2R1B Antibody [NBP3-37951] - Immunofluorescence analysis of MCF7 cells using [KO Validated] PPP2R1B Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.
PPP2R1B Antibody

Western Blot: PPP2R1B Antibody [NBP3-37951] -

Western Blot: PPP2R1B Antibody [NBP3-37951] - Western Blot analysis of lysates from wild type(WT) and PPP2R1B knockout (KO) hek293T(KO) cells, using [KO Validated] PPP2R1B Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for PPP2R1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPP2R1B

The PPP2R1B gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric co

Alternate Names

MGC26454, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PP2A, subunit A, PR65-beta isoform, PP2A, subunit A, R1-beta isoform, PP2A-Abeta, PR65B, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform, protein phosphatase 2, regulatory subunit A, beta, protein phosphatase 2, structural/regulatory subunit A, beta, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A betaisoform

Gene Symbol

PPP2R1B

Additional PPP2R1B Products

Product Documents for PPP2R1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPP2R1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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