Pref-1/DLK1/FA1 Antibody (3A10)
Novus Biologicals | Catalog # NBP2-37548
![Western Blot: Pref-1/DLK1/FA1 Antibody (3A10)BSA Free [NBP2-37548]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-3A10-Western-Blot-NBP2-37548-img0002.jpg "Western Blot: Pref-1/DLK1/FA1 Antibody (3A10)BSA Free [NBP2-37548]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3A10
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Product Specifications
Immunogen
Purified recombinant fragment of human Pref-1/DLK1/FA1 expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pref-1/DLK1/FA1 Antibody (3A10)
Western Blot: Pref-1/DLK1/FA1 Antibody (3A10)BSA Free [NBP2-37548]
Western Blot: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)![Immunocytochemistry/ Immunofluorescence: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-3A10-Immunocytochemistry-Immunofluorescence-NBP2-37548-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]")
Immunocytochemistry/ Immunofluorescence: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]
Immunocytochemistry/Immunofluorescence: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.![Flow Cytometry: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-3A10-Flow-Cytometry-NBP2-37548-img0004.jpg "Flow Cytometry: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]")
Flow Cytometry: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]
Flow Cytometry: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Flow cytometric analysis of NIH/3T3 cells using DLK1 mouse mAb (green) and negative control (red).![ELISA: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-3A10-ELISA-NBP2-37548-img0001.jpg "ELISA: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]")
ELISA: Pref-1/DLK1/FA1 Antibody (3A10) - BSA Free [NBP2-37548]
ELISA: Pref-1/DLK1/FA1 Antibody (3A10) [NBP2-37548] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Applications for Pref-1/DLK1/FA1 Antibody (3A10)
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pref-1/DLK1/FA1
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Alternate Names
DLK-1, DLK1, FA1, pG2, Pref1, ZOG
Gene Symbol
DLK1
Additional Pref-1/DLK1/FA1 Products
Product Documents for Pref-1/DLK1/FA1 Antibody (3A10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pref-1/DLK1/FA1 Antibody (3A10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Notch Signaling Pathways
