Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80046
![Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3)Azide and BSA Free [NBP2-80046]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-PF13-3-Western-Blot-NBP2-80046-img0003.jpg "Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3)Azide and BSA Free [NBP2-80046]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PF13-3
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human Pref-1/DLK1/FA1 (extracellular domain).
Specificity
Recognizes human Pref-1/DLK1/FA1. Does not cross-react with mouse Pref-1/DLK1/FA1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free
Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3)Azide and BSA Free [NBP2-80046]
Western Blot: Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - Analysis of human DLK-1 [Pref-1] using anti-DLK1 (human), mAb (PF13-3) at 1: 2,000 dilution. Panel A. 1. Human DLK-1-Fc protein. 2. Transfected human DLK-1 full length cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate.Panel B. 1. Human DLK-1-Fc protein. 2. Mouse DLK-1-Fc protein. 3. Human DNER-Fc protein. 4. Human DLL1-Fc protein. 5. Human DLL3-Fc protein. 6. Human DLL4-Fc protein. 7. Human Jagged1-Fc protein. 8. Human FGF23-Fc protein.![Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free [NBP2-80046]](https://resources.rndsystems.com/images/products/Pref-1-DLK1-FA1-Antibody-PF13-3-Immunohistochemistry-Paraffin-NBP2-80046-img0001.jpg "Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free [NBP2-80046]")
Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free [NBP2-80046]
Immunohistochemistry-Paraffin: Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - Immunohistochemical staining of DLK1 using anti-DLK1 (human), mAb (PF13-3) in human adrenal tissue (10 ug/mL). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Pref-1/DLK1/FA1 Antibody (PF13-3) [NBP2-80046] - FACS: DLK1-transfected HEK 293 (Full length) cells were stained significantly using anti-DLK1 (human), mAb (PF13-3).
Applications for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:2000 - 1:10000
Flow Cytometry
1:100 - 1:500
Immunohistochemistry
10 ug/mL
Immunohistochemistry-Paraffin
10 ug/mL
Western Blot
1:2000 - 1:10000
Application Notes
Western blot: 1:2000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST
with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom
temperature.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pref-1/DLK1/FA1
Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Alternate Names
DLK-1, DLK1, FA1, pG2, Pref1, ZOG
Gene Symbol
DLK1
Additional Pref-1/DLK1/FA1 Products
Product Documents for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pref-1/DLK1/FA1 Antibody (PF13-3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Notch Signaling Pathways
