ProDots Recombinant Human FGF basic (146 aa) Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD233-10
PRD233-50
PRD233-01
ProDots Recombinant Human FGF basic (146 aa) Protein Bioactivity
2 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

ProDots Recombinant Human FGF basic (146 aa) Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.

The ED50 for this effect is 0.1-0.6 ng/mL.

Source
E. coli-derived human FGF basic protein
Pro143 - Ser288
Accession #
N-terminal Sequence
Analysis
Ala-Pro143
Predicted Molecular Mass
16.5 kDa
SDS-PAGE

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Product Datasheets

PRD233

Reconstitution

For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human FGF basic (146 aa) Protein Bioactivity View Larger

ProDots® Recombinant Human FGF basic stimulates cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.

SDS-PAGE ProDots Recombinant Human FGF basic (146 aa) Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human FGF basic was resolved with SDS-PAGE under reducing (R) and non-reducing(NR) conditions and visualized with silver staining, showing a single band at 17 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FGF basic

FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.

Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin

FAQs

  1. What receptors does FGF basic bind?

  2. Does human FGF basic show activity on mouse cells?
    • Yes, it does. The bioassay uses NR-6 mouse fibroblast cells.  There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for ProDots Recombinant Human FGF basic (146 aa) Protein

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used ProDots Recombinant Human FGF basic (146 aa) Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


ProDots Recombinant Human FGF basic (146 aa) Protein
By Anonymous on 05/17/2016

Excellent product. Very easy to resuspend and use.