Prohibitin Antibody (3F4-2B2) - Azide and BSA Free

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2 Analysis of PHB expression in Hela.

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in PC-12.

Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 5 ug/ml.

Sandwich ELISA: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Detection limit for recombinant GST tagged PHB is approximately 0.03ng/ml as a capture antibody.

Western Blot: Prohibitin Antibody (3F4-2B2) - Azide and BSA Free [H00005245-M01] -

Comprehensive analysis of MSC-derived SIRP-EV.A–C Protein profile comparisons and reproducibility between C-EVs and SIRP-EVs. A Scatter plot consistency and R2 values for protein identification across replicates; Venn diagram overlaps of EV type-specific proteins. B Correlation coefficient analysis of protein profile similarities between replicates. C Quantification of SIRP alpha overexpression in SIRP-EVs compared to C-EVs. D Protein expression of EVs to examine SIRP alpha, EV markers (CD81, CD9, CD63, and TSG101), and non-EV marker, prohibitin. E Representative cryo-TEM image of EVs. F Size distribution of EVs analyzed by DLS. G Zeta potential measurements of EVs analyzed using NTA, which denote the surface charge distribution (left, n = 3). MemGlow staining of EVs (right). H Network diagram displaying over-represented Gene Ontology Biological Processes (GOBPs) among the top 506 SIRP-EV proteins. Analysis shows the proportion of these proteins compared to the total, with significant GOBPs (P < 0.05) indicated by black and red lines for group and individual function significance, respectively. I Quantification of cell binding, following a 30-min co-incubation of Cy5.5-labeled C-EV and SIRP-EV with CD47 KO and CD47 WT cells (n = 3). Bar graph data are presented as mean +/- SD. Statistical significance was determined by two-tailed unpaired Student’s t test (G), two-way ANOVA with Sidak’s post hoc test by comparing the groups treated with the same concentration of EVs (I). ****P < 0.0001. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39988725), licensed under a CC-BY license. Not internally tested by Novus Biologicals.