Prohibitin Antibody (5H7)

Novus Biologicals | Catalog # NBP2-37563

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H7
View all Prohibitin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Prohibitin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Prohibitin Antibody (5H7)

Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (5H7) [NBP2-37563] - Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PHB mouse mAb with DAB staining.
Flow Cytometry: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Flow Cytometry: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Flow Cytometry: Prohibitin Antibody (5H7) [NBP2-37563] - Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7)BSA Free [NBP2-37563]

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] - Western blot analysis using PHB mAb against human PHB (AA: 68-259) recombinant protein. (Expected MW is 46.7 kDa)
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

Immunohistochemistry-Paraffin: Prohibitin Antibody (5H7) [NBP2-37563] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PHB mouse mAb with DAB staining.
ELISA: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

ELISA: Prohibitin Antibody (5H7) - BSA Free [NBP2-37563]

ELISA: Prohibitin Antibody (5H7) [NBP2-37563] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Prohibitin Antibody (5H7)

Western Blot: Prohibitin Antibody (5H7) [NBP2-37563] -

Unlike mitoNEET, CISD2 protein level is moderately affected by iron chelator treatments. HeLa cells were treated with either SIH or DFO for the indicated times. Untreated cells are noted NT. MitoNEET and CISD2 protein levels were determined by immunoblotting using anti-CISD2 antibodies. Actin (cytosolic protein) and prohibitin (mitochondrial inner membrane-localized protein) were used as protein loading controls. The experiment was performed twice; one representative experiment is shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33916457), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Prohibitin Antibody (5H7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is a 30 kD member of the band 7 family containing a SPFH domain. Prohibitin is localized the nucleus and mitochondrial inner cell membrane. This protein functions as a negative regulator of cell proliferation associated with senescence and cell death and a transcriptional repressor via recruitment of Brg-1, Brm or N-CoR, or HDAC1. Prohibitin can also function as a membrane-bound chaperone for the assembly of the mitochondrial respiratory chain complex. Prohibitin is induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins. Prohibitin can be modified by phosphorylation and ubiquitination. This protein has been shown to interact with Rb, Brg-1, Brm, N-CoR, HDAC1, and PHB2.

Alternate Names

PHB

Gene Symbol

PHB1

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (5H7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Prohibitin Antibody (5H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

Citations for Prohibitin Antibody (5H7)

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Protocols

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