Proprotein Convertase 9/PCSK9 Antibody
Novus Biologicals | Catalog # NB300-959
![Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]](https://resources.rndsystems.com/images/products/Proprotein-Convertase-9-PCSK9-Antibody-Western-Blot-NB300-959-img0005.jpg "Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence CRSRHLAQASQELQ corresponding to C-Terminus according to NP_777596.2.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 29593095).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Proprotein Convertase 9/PCSK9 Antibody
Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]
Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]](https://resources.rndsystems.com/images/products/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-Negative-Control-showing-staining-of-paraffin-embedded-Human-Liver-with-no-primary-antibody--2932023925404.jpg "Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]")
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]](https://resources.rndsystems.com/images/products/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-5ug-ml-staining-of-paraffin-embedded-Human-Liver-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254023.jpg "Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]")
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959]
Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Proprotein Convertase 9/PCSK9 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunohistochemistry-Paraffin
5-6 ug/ml
Peptide ELISA
detection limit 1:16000
Western Blot
0.3-1 ug/ml
Application Notes
ICC/IF reactivity reported in (PMID: 30210081), Chip reactivity reported in (PMID: 20427574).
Reviewed Applications
Read 1 review rated 4 using NB300-959 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Proprotein Convertase 9/PCSK9
Alternate Names
FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9
Entrez Gene IDs
255738 (Human)
Gene Symbol
PCSK9
UniProt
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Product Documents for Proprotein Convertase 9/PCSK9 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Proprotein Convertase 9/PCSK9 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Proprotein Convertase 9/PCSK9 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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