Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free

Novus Biologicals | Catalog # NBP2-52539

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8A7C7

Format

BSA Free
View all Protein Phosphatase 1 beta Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Protein Phosphatase 1 beta (AA: 174-327) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis using Protein Phosphatase 1 beta mouse mAb against Jurkat (1), A431 (2), Hela (3), HepG2 (4), HEK293 (5), MCF-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunocytochemistry/ Immunofluorescence: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunocytochemistry/Immunofluorescence: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis of MCF-7 cells using PPP1CB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis of paraffin-embedded rectum cancer tissues using PPP1CB mouse mAb with DAB staining.
Flow Cytometry: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Flow Cytometry: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Flow Cytometry: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis of Jurkat cells using PPP1CB mouse mAb (green) and negative control (red).
Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis using PPP1CB mAb against HEK293 (1) and PPP1CB (AA: 174-327)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7)BSA Free [NBP2-52539]

Western Blot: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis using PPP1CB mAb against human PPP1CB (AA: 174-327) recombinant protein. (Expected MW is 43.2 kDa)
Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

Immunohistochemistry-Paraffin: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Analysis of paraffin-embedded cervical cancer tissues using PPP1CB mouse mAb with DAB staining.
ELISA: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

ELISA: Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free [NBP2-52539]

ELISA: Protein Phosphatase 1 beta Antibody (8A7C7) [NBP2-52539] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Phosphatase 1 beta

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favoring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Alternate Names

EC 3.1.3.16, EC 3.1.3.53, MGC3672, PP1beta, PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform, PPP1CD, protein phosphatase 1, catalytic subunit, beta isoform, protein phosphatase 1, catalytic subunit, beta isozyme, protein phosphatase 1-beta, protein phosphatase 1-delta, serine/threonine protein phosphatase PP1-beta catalytic subunit, serine/threonine-protein phosphatase PP1-beta catalytic subunit

Gene Symbol

PPP1CB

Additional Protein Phosphatase 1 beta Products

Product Documents for Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Protein Phosphatase 1 beta Antibody (8A7C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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