Prox1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-18605

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Prox1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Prox-1

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27122034).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Prox1 Antibody - Azide and BSA Free (NBP1-18605) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Prox1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Prox1 Antibody - Azide and BSA Free

Western Blot: Prox1 Antibody [NBP1-18605]

Western Blot: Prox1 Antibody [NBP1-18605]

Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1
Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605]

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605]

Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18605-img0008.jpg
Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605]

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605]

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.
Prox1 Antibody

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] -

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Prox1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:750 - 1:1000

Western Blot

1-5 ug/ml
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID 27122034). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 31696318).

Reviewed Applications

Read 1 review rated 3 using NBP1-18605 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

0.5X PBS, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prox1

Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues and may play a fundamental role in early development of the CNS. It may regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.

Long Name

Prospero-related Homeobox 1

Alternate Names

Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1

Entrez Gene IDs

5629 (Human)

Gene Symbol

PROX1

UniProt

Q92786

Additional Prox1 Products

Product Documents for Prox1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Prox1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Prox1 Antibody - Azide and BSA Free

Customer Reviews for Prox1 Antibody - Azide and BSA Free (1)

3 out of 5
1 Customer Rating
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100%
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1 Stars
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Customer Images

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 11/06/2014
    Human skin stained with prox1 (red), VEGFR3 (green) and Topro (nuclei)
    Prox1 Antibody - Azide and BSA Free NBP1-18605

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Protocols

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