PRPF8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93689

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRPF8 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3). MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

274 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRPF8 Antibody - BSA Free

Western Blot: PRPF8 AntibodyBSA Free [NBP2-93689]

Western Blot: PRPF8 AntibodyBSA Free [NBP2-93689]

Western Blot: PRPF8 Antibody [NBP2-93689] - Analysis of extracts of various cell lines, using PRPF8 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunocytochemistry/Immunofluorescence: PRPF8 Antibody [NBP2-93689] - Analysis of U-251MG cells using PRPF8. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded rat pancreas using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody - BSA Free [NBP2-93689]

Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-93689] - Immunohistochemistry of paraffin-embedded mouse brain using PRPF8 Rabbit pAb (NBP2-93689) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for PRPF8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRPF8

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Alternate Names

apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, pre-mRNA-processing-splicing factor 8, Prp8, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae), PRP8 pre-mRNA processing factor 8 homolog (yeast), PRP8,220 kDa U5 snRNP-specific protein, PRPC8HPRP8, RP13, Splicing factor Prp8, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Gene Symbol

PRPF8

Additional PRPF8 Products

Product Documents for PRPF8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRPF8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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