PSAP Antibody (4D5F4) - BSA Free

Novus Biologicals | Catalog # NBP2-37422

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D5F4

Format

BSA Free
View all PSAP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PSAP (AA: 325-524) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSAP Antibody (4D5F4) - BSA Free

Western Blot: PSAP Antibody (4D5F4)BSA Free [NBP2-37422]

Western Blot: PSAP Antibody (4D5F4)BSA Free [NBP2-37422]

Western Blot: PSAP Antibody (4D5F4) [NBP2-37422] - Western blot analysis using PSAP mAb against HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Immunocytochemistry/ Immunofluorescence: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Immunocytochemistry/Immunofluorescence: PSAP Antibody (4D5F4) [NBP2-37422] - Immunofluorescence analysis of HepG2 cells using PSAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Immunohistochemistry-Paraffin: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Immunohistochemistry-Paraffin: PSAP Antibody (4D5F4) [NBP2-37422] - Analysis of pancreas tissues using PSAP mouse mAb with DAB staining.
Flow Cytometry: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Flow Cytometry: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

Flow Cytometry: PSAP Antibody (4D5F4) [NBP2-37422] - Flow cytometric analysis of HeLa cells using PSAP mouse mAb (green) and negative control (purple).
Western Blot: PSAP Antibody (4D5F4)BSA Free [NBP2-37422]

Western Blot: PSAP Antibody (4D5F4)BSA Free [NBP2-37422]

Western Blot: PSAP Antibody (4D5F4) [NBP2-37422] - Western blot analysis using PSAP mAb against human PSAP recombinant protein. (Expected MW is 47.8 kDa)
ELISA: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

ELISA: PSAP Antibody (4D5F4) - BSA Free [NBP2-37422]

ELISA: PSAP Antibody (4D5F4) [NBP2-37422] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for PSAP Antibody (4D5F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSAP

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Long Name

Prosaposin

Alternate Names

GLBA, SAP1

Gene Symbol

PSAP

Additional PSAP Products

Product Documents for PSAP Antibody (4D5F4) - BSA Free

Certificate of Analysis

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Product Specific Notices for PSAP Antibody (4D5F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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