PSAP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83363

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSAP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSAP Antibody - BSA Free

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-83363 antibody. Corresponding PSAP RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: PSAP Antibody [NBP1-83363]

Immunocytochemistry/ Immunofluorescence: PSAP Antibody [NBP1-83363]

Immunocytochemistry/Immunofluorescence: PSAP Antibody [NBP1-83363] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363]

Immunohistochemistry-Paraffin: PSAP Antibody [NBP1-83363] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
PSAP Antibody - BSA Free Western Blot: PSAP Antibody - BSA Free [NBP1-83363]

Western Blot: PSAP Antibody - BSA Free [NBP1-83363]

Analysis in human cell lines SK-MEL-30 and HEK293 using Anti-PSAP antibody. Corresponding PSAP RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Applications for PSAP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSAP

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Long Name

Prosaposin

Alternate Names

GLBA, SAP1

Gene Symbol

PSAP

Additional PSAP Products

Product Documents for PSAP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PSAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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