PSD-95 Antibody (6G6-1C9) - BSA Free

Novus Biologicals | Catalog # NB300-556

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Invertebrate, Primate

Cited:

Human, Mouse, Rat, Invertebrate, Mollusc

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 6G6-1C9

Format

BSA Free
View all PSD-95 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant rat PSD-95.

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 18182049). Primate reactivity reported in scientific literature (PMID: 20519524). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Marker

post-Synaptic Marker

Specificity

Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PSD-95 Antibody (6G6-1C9) - BSA Free

Western Blot: PSD-95 Antibody (6G6-1C9)BSA Free [NB300-556]

Western Blot: PSD-95 Antibody (6G6-1C9)BSA Free [NB300-556]

Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0025.jpg
Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

PSD-95-Antibody-6G6-1C9-Immunohistochemistry-Paraffin-NB300-556-img0024.jpg
Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated and cultured using the Primary Neuron Isolation Kit. At day 28, neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were probed with a PSD95 monoclonal antibody at a dilution of 1:500 for 2 hours at room temperature or overnight at 4C, washed with HBSS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at dilution of 1:500 for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.
Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).
Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).
Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).
PSD-95 Antibody (6G6-1C9) - BSA Free

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] -

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PSD-95 Antibody (6G6-1C9) - BSA Free

Western Blot: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] -

Western Blot: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PSD-95 Antibody (6G6-1C9) - BSA Free

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] -

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures.Double labeling with anti-TUJ1 (A) & anti-VAMP2 (B) demonstrates that VAMP2 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against synaptophysin (P38) (D) & VAMP2 (E) indicates that VAMP2 is colocalized with the presynaptic marker (F). Double labeling for PSD-95 (G) & VAMP2 (H) shows partial colocalization postsynaptic (I). Small, high-resolution pictures of single synapses from (F) & (I) are shown in the right part of these images, respectively. Scale bar: 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PSD-95 Antibody (6G6-1C9) - BSA Free

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] -

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures.(A) Beta-tubulin (green, anti-TuJ1) labeling of dendrite, after anti-GluA1 external epitope [66] labeling & subsequent plasma membrane permeabilization. Note punctate GluA1 [66] labeling along the dendrite. (B) Synaptophysin (P38, green) & GluA1 [66]. (C) Synaptophysin (green) & GluA2 [66]. (D) Labeling with anti-GluA1. (E) Labeling with anti-GluA1, but the antibody was preincubated with the peptide antigen before staining. (F) Labeling with anti-GluA2. (G) Labeling with anti-GluA2, but the antibody was preincubated with the peptide antigen before staining. (H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) & (I) showing postsynaptic labeling of GluA1. Scale bar (in A, valid for all images): 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PSD-95 Antibody (6G6-1C9) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

2 ug / test (100ul)

Immunocytochemistry/ Immunofluorescence

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:2000
Application Notes
WB: Detects an approx. 95 kDa protein and a slightly larger band in rat brain extracts. Flow usage was reported in scientific literature (PMID: 19187438). IHC usage was reported in scientific literature (PMID: 21289286). IHC-Fr usage was reported in scientific literature (PMID: 24875483).

Reviewed Applications

Read 1 review rated 5 using NB300-556 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

3.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSD-95

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Long Name

Postsynaptic Density Protein 95/Disks Large Homolog 4

Alternate Names

DLG4, PSD95, SAP90

Entrez Gene IDs

1742 (Human); 13385 (Mouse); 29495 (Rat)

Gene Symbol

DLG4

Additional PSD-95 Products

Product Documents for PSD-95 Antibody (6G6-1C9) - BSA Free

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Product Specific Notices for PSD-95 Antibody (6G6-1C9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSD-95 Antibody (6G6-1C9) - BSA Free

Customer Reviews for PSD-95 Antibody (6G6-1C9) - BSA Free (1)

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  • PSD-95 Antibody (6G6-1C9)
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: rat cortical culture (3 weeks old)
    Species: Rat
    Verified Customer | Posted 06/27/2017
    Mixed rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected.
    Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT Blocking Solution & Duration: 10% donkey serum in PBS with 0.1% triton, 30 minutes RT Primary Antibody Diluent and Dilutions Tested: 1:650 in 5% donkey serum in PBS, over night 4°C Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse Alexa647, 1:1250 in PBS, 2 hours, RT
    PSD-95 Antibody (6G6-1C9) - BSA Free NB300-556

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