PSD-95 Antibody (7E3) - BSA Free

Novus Biologicals | Catalog # NBP2-12872

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Microarray

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E3

Format

BSA Free
View all PSD-95 Primary Antibodies »

Product Specifications

Immunogen

Recombinant rat PSD-95

Localization

Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density, Cell Projection, Axon

Marker

post-Synaptic Marker

Specificity

Detects ~100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PSD-95 Antibody (7E3) - BSA Free

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872]

Western Blot: PSD-95 Antibody (7E3) [NBP2-12872] - Western Blot analysis of Rat brain membrane lysate showing detection of PSD-95 protein using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:1000.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filamentous-like staining.
Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Immunohistochemistry analysis using Mouse Anti-PSD-95 Monoclonal Antibody, Clone 7E3 (NBP2-12872). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-PSD-95 Monoclonal Antibody (NBP2-12872) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, some hair follicle staining, dermal staining. Backskin obtained from transgenic mice..
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872]

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain cultured neurons. Image from verified customer review.
Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry: PSD-95 Antibody (7E3) [NBP2-12872] - Tissue: Neocortex. Species: Rat. Primary Antibody: Mouse Anti-PSD95 Monoclonal Antibody at 1:1000.
Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872]

Immunohistochemistry-Frozen: PSD-95 Antibody (7E3) [NBP2-12872] - Mouse brain section (hippocampus). Image from verified customer review.
PSD-95 Antibody (7E3) - BSA Free

Western Blot: PSD-95 Antibody (7E3) - BSA Free [NBP2-12872] -

Microglial cells and SYN/PSD95 protein in the hippocampal region of HO rats. (A) Immunofluorescence staining of Iba1+ cells. Iba1 (green) and DAPI (blue). Scale bars, 100 μm for low magnification images and 50 μm for high magnification images; quantification of Iba1+ cells and the cell body area in the hippocampus, n = 4–6, **P < 0.01 vs. sham-operated group. (B) Immunofluorescence staining of Iba1+ and SYN in the hippocampus: SYN (red), Iba1 (green), and DAPI (blue); scale bars, 50 μm, n = 6, **P < 0.01 vs. sham-operated group. (C) Immunofluorescence staining of Iba1+ and PSD95 in the hippocampus: PSD95 (red), Iba1 (green), and DAPI (blue); scale bars, 50 μm, n = 6, **P < 0.01 vs. sham-operated group. (D–F) The protein levels of SYN and PSD95 in the hippocampus, n = 3, *P < 0.05 vs. sham-operated group. All data are presented as the M +/- SEM of each group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36072565), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PSD-95 Antibody (7E3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/mL was sufficient for detection of PSD-95 on 20 ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Use in IHC-Fr reported by customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-12872 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSD-95

1ug/ml was sufficient for detection of PSD-95 on 20ug rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse igG: HRP as the secondary.

Long Name

Postsynaptic Density Protein 95/Disks Large Homolog 4

Alternate Names

DLG4, PSD95, SAP90

Gene Symbol

DLG4

UniProt

P31016 (Rat)

Additional PSD-95 Products

Product Documents for PSD-95 Antibody (7E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for PSD-95 Antibody (7E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSD-95 Antibody (7E3) - BSA Free

Customer Reviews for PSD-95 Antibody (7E3) - BSA Free (1)

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  • PSD-95 Antibody (7E3)
    Name: Xuecheng Qiu
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain and Cell culture
    Species: Mouse
    Verified Customer | Posted 05/12/2019
    PSD-95 Antibody (7E3) - BSA Free NBP2-12872

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