PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Novus Biologicals | Catalog # NB100-78039

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Kappa Clone # HECA-452

Format

BSA Free
View all PSGL-1/CD162 Primary Antibodies »

Product Specifications

Immunogen

Human tonsil stroma

Specificity

CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).

Clonality

Monoclonal

Host

Rat

Isotype

IgM Kappa

Scientific Data Images for PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified customer review.
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC.
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarcinoma. Note:- Besides lymphocytes, CLA has been shown to be present on cells of normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).
Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat-Dylight 488 conjugated secondary antibody. A co-stain was performed using CD14 antibody NB100-77758AF488.
PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Western Blot: PSGL-1/CD162 Antibody (HECA-452) - BSA Free [NB100-78039] -

High level of P-selectin leads to increased PSGL-1 expression and NETs formation in human neutrophils. Human peripheral blood neutrophils were isolated and treated with P-selectin recombinant protein at concentrations of 10, 20 and 50 nM. (A) Representative images of neutrophil stained with PSGL-1 (yellow) and DAPI (blue). The inset box from each group is magnified. Scale bar: 5 μm and 20 μm, respectively. (B) Cell lysates were collected and subjected to Western blot analysis for PSGL-1. GAPDH was used as a loading control. Data from one representative experiment are shown. (C) Relative intensities of PSGL-1 against GAPDH. (n=3, **P < 0.01) (D) Representative images of neutrophils stained with MPO (red), CitH3 (Green) and DAPI (blue). The inset box from each group is magnified. Scale bar: 50 μm and 20 μm, respectively. The arrows indicate NETs. (E) NET-forming cells per field are quantified. (n=6, ****P < 0.0001) The neutrophil supernatants were assessed for cf-DNA (F) and MPO-DNA complexes (G). (n=3, *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37841279), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Application
Recommended Usage

Flow Cytometry

< = 2 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 1693467)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-78039 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Certificate of Analysis

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Product Specific Notices for PSGL-1/CD162 Antibody (HECA-452) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSGL-1/CD162 Antibody (HECA-452) - BSA Free

Customer Reviews for PSGL-1/CD162 Antibody (HECA-452) - BSA Free (1)

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Customer Images

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  • PSGL-1/CD162 Antibody (HECA-452)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 10/13/2021
    5ug/mL, stained with Leica Refine DAB kit
    PSGL-1/CD162 Antibody (HECA-452) - BSA Free NB100-78039

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